| |

Beginning March 20th FedEx will provide carrier services for all domestic shipments. There will be no changes to service levels or NEB's Packaging, Shipping, and Delivery Policy.

Home Restriction Endonucleases Products TspMI

TspMI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence C^CCGG_G

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Restriction Enzyme Cut Site: C/CCGGG

Catalog #	Concentration	Size	List Price	Your Price
R0709S	5,000 units/ml	200 units	$77.00	Sign In Sign In

Please enter a quantity for at least one size

Product Information

Product Source

Thermus species (P. Sharma)

This product is related to the following categories:: Restriction Endonucleases T Z,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0709S -20

TspMI R0709SVIAL -20 1 x 0.04 ml 5,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pBC4 DNA in 1 hour at 75°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 75°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 75%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Buffer

20 mM Tris-HCl
300 mM NaCl
1 mM DTT
1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.1% Triton® X-100
pH 8 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Isoschizomers

Cfr9I
SmaI
XmaI

Activity at Temperature
@37°C: 10%
@65°C: 100%
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. May exhibit star activity in NEBuffer r1.1, NEBuffer r2.1 or NEBuffer r 3.1.
2. Temperature activity curve of activity:
  37°C gives 10% optimal activity
  65°C gives 100% optimal activity
  75°C gives 100% optimal activity
3. TspMI is an isoschizomer of XmaI.
4. For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
5. Blocked by CpG methylation.