Monarch® DNA Gel Extraction Kit

Learn more about Monarch Nucleic Acid Purification Kits

Quickly and easily purify DNA from agarose gels with high yields.

  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carry-over with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits 
  • Responsibly-sourced and recyclable packaging

Ordering Information

T1020S_pdp_800X450
  • 50 preps
    $99.00
  • 250 preps
    $455.00
  • Product Information

    The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging


    View our videos on protocols, tips, and recycling Monarch.

    Monarch DNA Cleanup Column Design
    Monarch DNA Cleanup Column Design
    Monarch columns are designed for performance
    Monarch columns are designed for performance. Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
    Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.

    Advantages:
    • Elute in as little as 6 μl
    • Prevent buffer retention and salt carry-over with optimized column design
    • Save time with fast, user-friendly protocols
    • No need to monitor pH or add isopropanol
    • Buffers and columns available separately
    • Significantly less plastic used when compared with other kits
    • Responsibly-sourced and recyclable packaging

    Specifications

    DNA Sample Type: double-stranded DNA from agarose gels
    Binding Capacity: up to 5 μg
    DNA Size Range: ~50 bp to 25 kb
    Typical Recovery: DNA (50 bp to 10 kb): 70–90%
    DNA (11–23 kb): 50–70%
    Elution Volume: ≥ 6 μl
    Purity: A260/280 > 1.8
    Protocol Time: 10 minutes of spin and incubation time
    Compatible Downstream
    Applications:
    ligation, restriction digestion, labeling and other enzymatic
    manipulations, library construction and DNA sequencing.

    Monarch DNA Gel Extraction Kit Protocol 
    Monarch DNA Gel Extraction Kit Protocol
    DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications
     DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications. One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume.
    One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. 
    Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. 
    Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights. A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison.
    A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. 
    DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.
    DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367 (https://www.neb.com/products/m0367-blunt-ta-ligase-master-mix) ). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232 (https://www.neb.com/products/n3232-1-kb-dna-ladder) ).
    Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).

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    Kit Components

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    Monarch® DNA Elution Buffer 25 1 X
    Monarch® Gel Dissolving Buffer 25 1 X
    Monarch® DNA Wash Buffer 25 5 X
    Monarch® DNA Cleanup Columns (5 μg) 25
    Product Categories:
    DNA Gel Purification Products,
    Nucleic Acid Purification Products
    Applications:
    Nucleic Acid Purification
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