A new version of this kit is now available, featuring upgraded spin columns. Migrate to the new Monarch Spin DNA Gel Extraction Kit (NEB #T1120).
Catalog # T1020 was discontinued on October 22, 2024
Quickly and easily purify DNA from agarose gels with high yields.
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
Product Information
The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
Monarch DNA Cleanup Column Design Monarch columns are designed for performance Monarch columns are designed without a frit, which eliminates buffer retention and the risk of carryover contamination, providing fast, worry-free DNA purification.
Advantages:
Elute in as little as 6 μl
Prevent buffer retention and salt carry-over with optimized column design
Save time with fast, user-friendly protocols
No need to monitor pH or add isopropanol
Buffers and columns available separately
Significantly less plastic used when compared with other kits
Responsibly-sourced and recyclable packaging
Specifications
DNA Sample Type:
double-stranded DNA from agarose gels
Binding Capacity:
up to 5 μg
DNA Size Range:
~50 bp to 25 kb
Typical Recovery:
DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
Elution Volume:
≥ 6 μl
Purity:
A260/280 > 1.8
Protocol Time:
10 minutes of spin and incubation time
Compatible Downstream
Applications:
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
Monarch DNA Gel Extraction Kit Protocol DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications
One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights.
A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved on a new gel with the remainder of the original mixture for comparison. DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated.
Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised, and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total) was ligated using the Blunt/TA Ligase Master Mix (NEB #M0367). Representative samples from 5 replicates were resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder (NEB #N3232).
This product is related to the following categories:
The kit should be stored at room temperature. Always keep buffer bottles tightly closed and keep columns sealed in the enclosed zip-lock bag. For information regarding the composition of buffers, please consult the Safety Data Sheets. Proper laboratory safety practices should be employed, including the use of lab coats, gloves and eye protection.
Reagents added incorrectly. Check protocol to ensure correct buffer reconstitution, order of addition for buffers and proper handling of column flow-through and eluents.
Gel slice not fully dissolved. Small clumps of agarose may clog the column or interfere with DNA binding. Be sure to incubate the gel slice in the Monarch Gel Dissolving Buffer for the specified time and within the proper temperature range. Mix the sample and inspect periodically to monitor dissolution of the agarose.
Gel dissolved above 60°C. The DNA may become denatured if incubated at higher temperatures than the specified range of 37–55°C.
Incomplete elution during prep. Ensure the DNA Elution Buffer is delivered directly to the center of the column so that the matrix is completely covered and elution is efficient. Larger elution volumes and longer incubation times can increase yield of DNA off the column at the cost of dilution of the sample and increased processing times. For typical fragments below 10 kb, the recommended elution volumes and incubation times should be sufficient, unless the maximal yield is desired. For the purification of larger fragments, heating the DNA Elution Buffer to 50°C prior to eluting and extending the incubation time after buffer addition to 5 minutes can improve yield. Additionally, multiple rounds of elution can be employed to increase the amount of DNA eluted, at the expense of dilution of the sample.
Low DNA Performance
Gel slice not fully dissolved. Undissolved agarose may leach salts into the eluted DNA. Be sure to incubate the gel slice and the Monarch Gel Dissolving Buffer mixture for the specified time and temperature. Mix the sample and inspect periodically to monitor dissolving of the agarose.
Ethanol has been carried over. Ensure final wash spin time is 1 minute to ensure complete removal of the wash buffer from the column and be careful when transferring the column to a new tube for elution step to ensure column tip does not contact column flow-through.
Trace amounts of salts that produce low OD260/230 ratios can also be carried over during the elution step. Be careful when transferring column to new tube for elution step to ensure the column tip does not contact column flow-through.
Wang M, Wang B, Jiang K, Liu M, Shi X, Wang L. (2018) A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri Fish Shellfish Immunol; PubMedID: 29127027
Kose, S. H., Grice, K., Orsi, W. D., Ballal, M., and Coolen, M. J. L. (2018) Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria Sci Rep;
Bornikoel J, Staiger J, Madlung J, Forchhammer K, Maldener I. (2018) LytM factor Alr3353 affects filament morphology and cell-cell communication in the multicellular cyanobacterium Anabaena sp. PCC 7120 Mol Microbiol; PubMedID: 29437253
Kang B, Peng B, Wu H, Liu L, Wu W, Gu Q. (2018) Host-associated selection of a P3 mutant of zucchini yellow mosaic virus affects viral infectivity in watermelon Arch Virol; PubMedID: 29426994
Perniss A, Schmidt N, Gurtner C, Dietert K, Schwengers O, Weigel M, Hempe J, Ewers C, Pfeil U, Gärtner U, Gruber AD, Hain T, Kummer W (2018) Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice Sci Rep; 8(1):5681, DOI: 10.1038/s41598-018-23830-4
Roussy M, Bilodeau M, Jouan L, Tibout P, Laramée L, Lemyre E, Cardin S, Sauvageau C, Couture F, Choblet A, Patey N, Gendron P, Duval M, Teira P, Hébert J, Wilhelm BT, Choi JK, Gruber TA, Bittencourt H, Cellot S. (2018) NUP98-BPTF gene fusion identified in primary refractory acute megakaryoblastic leukemia of infancy Genes Chromosomes Cancer; PubMedID: 29427526
Hua Yang, Silvia Jenni, Milena Colovic, Helen Merkens, Carlee Poleschuk, Isabel Rodrigo, Qing Miao, Bruce F. Johnson4, Michael J. Rishel4, Vesna Sossi, Jack M. Webster, François Bénard and Paul Schaffer (2017) F-5-Fluoroaminosuberic Acid as a Potential Tracer to Gauge Oxidative Stress in Breast Cancer Models. J Nucl Med; PubMedID: 5331935
Zhang Y, Tanner N. (2017) Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein BioTechniques; 1–8, PubMedID: 28819114, DOI: 10.2144/btn-2024-0012
Adam Waalkes, Kelsi Penewit, Brent L. Wood, David Wu, and Stephen J. Salipante (2017) Ultrasensitive detection of acute myeloid leukemia minimal residual disease using single molecule molecular inversion probes. Haematologica; PubMedID: 28572161
Hannah G. Reich, Deborah L. Robertson, Gretchen Goodbody-Gringley (2016) Do the shuffle: Changes in Symbiodinium consortia throughout juvenile coral development. PLOS One; PubMedID: 28182684
Publications
Zhang Y, Tanner N. (2017) Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein BioTechniques; 1–8, PubMedID: 28819114, DOI: 10.2144/btn-2024-0012
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