rCutSmart™

Simplify Reaction Setup and Double Digestion with rCutSmart Buffer™

Over 210 restriction enzymes are 100% active in a single buffer, rCutSmart Buffer, making it significantly easier to set up your double digest reactions.

Since rCutSmart Buffer includes Recombinant Albumin, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in rCutSmart Buffer, eliminating the need for subsequent purification. 

View components of old and new buffers side by side.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Helpful Resources:

• NEBuffer Activity/Performance Chart with Restriction Enzymes 
• Activity of DNA Modifying Enzymes in rCutSmart^® Buffer
• View the NEBuffer Comparison Chart to compare the components and the concentration of each component in our restriction enzyme buffers
• REBASE^®