Beginning in May 2021, we will be gradually transitioning the Monarch DNA Cleanup Binding Buffer to a concentrated format which requires the addition of isopropanol by the user. The protocols on the website have been updated to reflect this change, but please refer to the instructions provided with your products, as your lot may not be affected.
Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.
View our videos on protocols, tips, and recycling Monarch.
|PCR cleanup||DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc.|
|Enzymatic reaction cleanup||Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample.|
|cDNA cleanup||DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides.|
|Labeling cleanup||Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate|
|Plasmid cleanup||Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit.|
|Oligonucleotide cleanup||ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.|
|DNA Sample Type:||DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.
|Binding Capacity:||up to 5 μg|
|DNA Size Range:||~50 bp to 25 kb
DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
DNA (50 bp to 10 kb): 70–90%
|Elution Volume:||≥ 6 μl|
|Purity:||A260/280 > 1.8 and A260/230 > 1.8
|Protocol Time:||5 minutes of spin and incubation time|
|ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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