Time-Saver™ Qualified Restriction Enzymes
Choose Type:
- Do degenerate recognition sites need to be palindromic?
- How can I generate a restriction enzyme site map for my sequence?
- How can I search for a restriction enzyme by sequence, overhang or name?
- How should I stop my restriction digest?
- How stable is a particular restriction enzyme?
- I don't see any cleavage after my restriction digest. What factors can interfere with cleavage?
- Is extended digestion (incubation times > 1 hour) recommended?
- What does it mean to be Time-Saver™ qualified?
- What information is available in the Restriction Enzyme Database (REBASE)?
- When is star activity a concern?
- When should I choose the HF version of the enzyme?
- How should I set up a restriction digest?
- What does HF® refer to following the name of a restriction enzyme?
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Restriction Enzymes at NEB: Over 30 years of Innovation
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
- DNA Sequences and Maps Tool
- Alphabetized List of Recognition Sequences
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Multiple Recognition Sequences
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoschizomers
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Restriction Enzyme Troubleshooting Guide
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction of Foreign DNA by E. coli K-12
- Site Preferences
- Star Activity
Feature Articles
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Enzymes powerful enough to digest in 5-15 minutes
- Flexible enough for overnight incubation
- No special formulation
- No added expense
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.