BspQI-HF^®

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R3712S     -20       BspQI-HF® R3712SVIAL -20 1 x 0.05 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X
R3712L     -20       BspQI-HF® R3712LVIAL -20 1 x 0.25 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   Gel Loading Dye, Purple (6X) B7024AVIAL 25 1 x 0.5 ml 6 X

Companion Products

• NEBridge^® Golden Gate Assembly Kit (BsmBI-v2)
• NEBridge^® Golden Gate Assembly Kit (BsaI-HF^®v2)
• Monarch^® Spin Plasmid Miniprep Kit
• Monarch^® Spin DNA Gel Extraction Kit
• Monarch^® Spin PCR and DNA Cleanup Kit (5 μg)
• Nuclease-free Water

Materials Sold Separately

• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

1. BspQI-HF has the same specificity of BspQI (R0712), but it has been engineered for reduced star activity.
2. BspQI-HF is an isoschizomer of SapI.
3. Not Sensitive to CpG, dcm, or dam methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Alteration of Apparent Recognition Specificities Using Methylases
• Cleavage Close to the End of DNA Fragments
• Dam and Dcm Methylases of E. coli
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• General Molecular Biology Data
• Getting Started with Molecular Cloning: Simple Tips to Improve your Cloning Efficiency
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Restriction Enzymes for Droplet Digital PCR (ddPCR)
• Restriction enzymes requiring multi-sites for efficient cleavage
• Restriction of Foreign DNA by E. coli K-12
• Site Preferences
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Sequences
• Cleavage of Supercoiled DNA
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Time-Saver™ Qualified Enzymes
• Type IIS Restriction Enzymes
• Why Choose Recombinant Enzymes?

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. What is the difference between BspQI-HF and BspQI (R0712)?
2. Which restriction enzymes are used in Golden Gate Assembly?
3. Which restriction enzymes are used in GoldenBraid Assembly?
4. Does NEB offer any BSA-free and/or animal origin-free restriction enzymes for linearization of plasmids for mRNA vaccine development?
5. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
6. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
8. Can the change in buffer preference of the HF enzyme be advantageous?
9. Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?

• EpiMark® Methylated DNA Enrichment Kit Troubleshooting Guide
• Restriction Enzyme Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R3712S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R3712S_v1_10275071
• R3712L_v1_10275070
• R3712L_v1_10288306

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BspQI-HF^®
• rCutSmart™ Buffer
• Gel Loading Dye, Purple (6X)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.