Restriction Endonucleases

Restriction Endonucleases

The Leader in the Discovery and Production of Restriction Enzymes

With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.

For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.



All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details.

Convenience

  • >210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer.
  • 193 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from 283 restriction enzymes, the largest selection commercially available.

Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Loading Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

Use Enzyme Finder to select restriction enzyme by name, sequence, overhang or type. 

  1. NEB TV Episode 15

    These days, restriction enzymes are being used in many more applications other than cloning. Learn more in this episode of NEB TV.

  2. Type II Restriction Enzymes

    Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.

  3. Type I Restriction Enzymes

    Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.

  4. Type III Restriction Enzymes

    Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.

  5. The Interaction of Restriction Enzymes and DNA

    Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.

  6. Discovering New Restriction Enzymes

    Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

  7. Restriction Enzymes in Chromatin Conformation Capture

    Chromatin conformation capture (3C) techniques allow study of the spatial organization of eukaryotic chromosomes in a 3D context. Learn more about this and other applications of restriction enzymes.

  8. Restriction Enzymes in Droplet Digital PCR

    Droplet digital PCR is a method for accurately quantitating copies of DNA or RNA in a sample. Each PCR reaction is separated into thousands or millions of droplets for analysis. Learn more about droplet digital PCR.

  9. Restriction Enzymes in Golden Gate Assembly

    Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for reassembly. View a list of TypeIIS enzymes.

  10. Restriction Enzymes in Isothermal Amplification

    Isothermal amplification generates many copies of a target sequence in a short period of time, at a constant temperature. Learn more about isothermal amplification.

  11. Restriction Enzymes in Optical Mapping

    Optical mapping is a method that allows production of restriction maps of whole chromosomes or genomes. Learn more about optical mapping.



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Restriction Endonucleases includes these subcategories:

Restriction Endonucleases

    Publications related to Restriction Endonucleases:

  1. Fu YB, Peterson G. W., Dong Y (2016). Increasing Genome Sampling and Improving SNP Genotyping for Genotyping-by-Sequencing with New Combinations of Restriction Enzymes G3. 6:4, 845-846. PubMedID: 26818077
  2. Shah, S., Sanchez, J., Stewart, A., et al. (2015). Probing the Run-On Oligomer of Activated SgrAI Bound to DNA PLoS One. 10(4), PubMedID: 25880668, DOI: 10.1371/journal.pone.0124783.
  3. Loenen, W.A., Raleigh, E.A. (2014). The other face of restriction: modification-dependent enzymes. Nucleic Acids Res. 42, 56-69. PubMedID: 23990325
  4. Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. (2013). Massively parallel characterization of restriction endonucleases Scopus. 41(11), e119. PubMedID: 23605040, DOI: 10.1093/nar/gkt257