Restriction Endonucleases
  • My NEB
  • Print
  • PDF
  • Restriction Endonucleases

    The Leader in the Discovery and Production of Restriction Enzymes

    After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

    NEB scientists continue to improve its portfolio of restriction enzymes, as well as explore their utility in new technologies. As a result, NEB scientists continue to publish scientific papers and to be awarded grants in this area. With the industry’s largest research and development group dedicated to restriction enzymes, we are proud to have been there first: the first to commercialize a recombinant enzyme, the first to introduce a nicking enzyme, and the first to supply a true restriction enzyme master mix. In addition, NEB has an ongoing history of innovation by engineering restriction enzymes with altered specificities and improved performance. Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance.



    All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details.

    Convenience

    Performance

    • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer.
    • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

    Use Enzyme Finder to select restriction enzyme by name, sequence, overhang or type. 

    1. Cloning With Restriction Enzymes

      Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

    2. CutSmart Restriction Enzyme Buffer

      Over 200 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart™ Buffer and why it matters to you.

    3. What are Restriction Enzymes?

      Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

    4. Time-Saver Qualified Restriction Enzymes

      How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

    5. Standard Protocol for Restriction Enzyme Digests

      Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

    6. What is Restriction Enzyme Star Activity?

      Learn what Star Activity is, why it is detrimental to accurate restriction enzyme digestion, and how NEB's HF enzymes are engineered to avoid it.

    7. 6 Reasons to Try RE-Mix Restriction Enzyme Master Mixes

    8. Reduce Star Activity with High-Fidelity Restriction Enzymes

      NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

    9. The Interaction of Restriction Enzymes and DNA

      Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.

    10. Why is My Restriction Enzyme Not Cutting DNA?

      Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.

    11. Restriction Enzyme Digest Problem: Too Many DNA Bands

      Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.

    12. Discovering New Restriction Enzymes

      Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

    13. Setting-up Restriction Enzyme Digests with RE-Mix Master Mixes

      RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

    Restriction Endonucleases includes these subcategories:

    Restriction Endonucleases

      Publications related to Restriction Endonucleases:

    1. Loenen, W.A., Dryden, D.T., Raleigh, E.A., Wilson, G.G., Murray, N.E. (2014)Highlights of the DNA cutters: a short history of the restriction enzymes. Nucleic Acids Res 42, 3-19. PubMedID: 24141096
    2. Loenen, W.A., Raleigh, E.A. (2014)The other face of restriction: modification-dependent enzymes. Nucleic Acids Res 42, 56-69. PubMedID: 23990325
    3. Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. (2013)Massively parallel characterization of restriction endonucleases Scopus 41, e119. PubMedID: 23605040, DOI: 10.1093/nar/gkt257