High-Fidelity (HF®) Restriction Endonucleases
For over 45 years, New England Biolabs has been developing innovative solutions for molecular biology applications. The respected leader in the field of restriction enzyme biology, NEB has developed a line of High-Fidelity (HF®) Restriction Enzymes. These engineered enzymes have the same specificity as the native enzyme, with the added benefit of reduced star activity, rapid digestion (5-15 minutes) and 100% activity in rCutSmart™ and CutSmart® Buffer.
Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Optimized performance for a wide range of conditions
High Fidelity Restriction Enzymes have been engineered by exchanging functional amino acid residues and then screening for optimal mutants that perform under a wide range of conditions. Whether you are setting up digests for 5-15 minutes or overnight, or using varying amounts of enzymes, HF enzymes ensure the performance you need.
We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, NEB will be switching our current BSA-containing reaction buffers (NEBuffer™ 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin (rAlbumin)-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We anticipate that this switch may take as long as 6 months to complete. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. Find more details at www.neb.com/BSA-free.
During this transition period, you may receive product with BSA or rAlbumin-containing buffers. NEB has rigorously tested both and has not seen any difference in enzyme performance when using either buffer. Either buffer can be used with your enzyme. All website content will be switched in April to reflect the changes, although you may not receive the new buffer with your product immediately.
HF® is a registered trademark of New England Biolabs, Inc.
FastDigest® is a registered trademark of Fermentas.
Choose Type:
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Restriction Enzymes at NEB: Over 30 years of Innovation
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
- Competitor Cross-Reference Tools
- DNA Sequences and Maps Tool
- Enzyme Finder
- Alphabetized List of Recognition Specificities
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Enzymes with Multiple Recognition Sequences
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoschizomers
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Restriction Enzyme Troubleshooting Guide
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction of Foreign DNA by E. coli K-12
- Site Preferences
- Star Activity
Feature Articles
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. 2013. Massively parallel characterization of restriction endonucleases Scopus. 41(11), PubMedID: 23605040, DOI: 10.1093/nar/gkt257
- One buffer convenience with no loss of performance
- Reduced star activity eliminates unwanted cleavage
- Time-Saver qualified for 5-15 minute digests, flexible enough to digest overnight.
- Engineered for performance under a wide range of conditions
- Added flexibility without added cost
- Cloning
- Genotyping
- Mutational Analysis
- Mapping
- Probe Preparation
- Sequencing
- Methylation Detection
- Any application that requires high fidelity or flexible reaction setup
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.
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Discovering New Restriction Enzymes
Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.
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How do restriction enzymes interact with DNA?
Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data.
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Restriction Enzyme Digest Problem: Too Many DNA Bands
Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.
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What is Restriction Enzyme Star Activity?
Learn what Star Activity is, why it is detrimental to accurate restriction enzyme digestion, and how NEB's HF enzymes are engineered to avoid it.
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NEB® Restriction Enzyme Double Digest Protocol
Double digestions can save you time, and this video can offer tips for how to achieve the best results, no matter which of NEB's restriction enzymes you're using.
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
Learn more about what causes this common problem, and how NEB's enzymes are QC'd to avoid DNA smearing.
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Standard Protocol for Restriction Enzyme Digests
Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.
