Cut Smarter with Restriction Enzymes from NEB After nearly 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance. All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details. Convenience Over 200 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. Over 180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight. RE-Mix® Restriction Enzyme Master Mixes require only the addition of DNA and water – it’s that simple! Choose from 276 restriction enzymes, the largest selection commercially available. Performance Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency. Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.