Restriction Endonucleases

Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details. 

Convenience

  • A vial of 6X Purple Load Dye is included with most restriction enzymes.
  • Over 210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. 
  • >190 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from >280 restriction enzymes, the largest selection commercially available.

Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.

Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.

  1. Type II Restriction Enzymes

    Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.

  2. Type I Restriction Enzymes

    Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.

  3. Type III Restriction Enzymes

    Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.

  4. CutSmart Restriction Enzyme Buffer

    >210 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart® Buffer and why it matters to you.

  5. Time-Saver Qualified Restriction Enzymes

    How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

  6. Cloning With Restriction Enzymes

    Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

  7. Reduce Star Activity with High-Fidelity Restriction Enzymes

    NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

  8. Standard Protocol for Restriction Enzyme Digests

    Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

  9. 6 Reasons to Try RE-Mix Restriction Enzyme Master Mixes

  10. What are Restriction Enzymes?

    Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

  11. Why is My Restriction Enzyme Not Cutting DNA?

    Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.

  12. Restriction Enzyme Digest Problem: Too Many DNA Bands

    Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.

  13. Setting-up Restriction Enzyme Digests with RE-Mix Master Mixes

    RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

  14. Restriction Enzyme Digest Protocol: Cutting Close to DNA End

    When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.

Restriction Endonucleases

Restriction Endonucleases: A

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Restriction Endonucleases: B

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Restriction Endonucleases: C-G

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Restriction Endonucleases: H-M

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Restriction Endonucleases: N-O

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Restriction Endonucleases: P-R

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Restriction Endonucleases: S

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Restriction Endonucleases: T-Z

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HF®, Nicking, Time-Saver & Other

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Restriction Endonuclease Buffers & Diluents

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Restriction Endonucleases: A

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Restriction Endonucleases: B

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Restriction Endonucleases: C-G

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Restriction Endonucleases: H-M

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Restriction Endonucleases: N-O

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Restriction Endonucleases: P-R

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Restriction Endonucleases: S

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Restriction Endonucleases: T-Z

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HF®, Nicking, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

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Homing Endonucleases

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Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

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Time-Saver™ Qualified Restriction Enzymes

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Restriction Endonuclease Buffers & Diluents

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