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Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details. 

Convenience

  • A vial of 6X Purple Load Dye is included with most restriction enzymes.
  • Over 210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. 
  • >190 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from >280 restriction enzymes, the largest selection commercially available.



Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.


Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.

Restriction Endonucleases

Restriction Endonucleases: A

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Order AatII

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Order AbaSI

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AfeI

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Order AflII

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Order AflIII

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Order AgeI

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Order AgeI-HF®

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Order AhdI

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Order AleI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AsiSI

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Order AvaI

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Order AvaII

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Order AvrII

Restriction Endonucleases: B

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BanI

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Order BanII

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Order BbsI

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Order BbsI-HF®

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Order BbvCI

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Order BbvI

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Order BccI

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Order BceAI

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Order BcgI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfaI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmrI

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Order BmtI

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Order BmtI-HF®

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Order BpmI

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Order Bpu10I

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Order BpuEI

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Order BsaAI

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Order BsaBI

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Order BsaHI

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Order BsaI

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Order BsaI-HF®

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Order BsaJI

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BseYI

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Order BsgI

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Order BsiEI

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Order BsiHKAI

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Order BsiWI

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Order BsiWI-HF®

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmFI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspDI

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Order BspEI

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Order BspHI

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Order BspMI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrFαI

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Order BsrGI

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Order BsrGI-HF®

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Order BsrI

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Order BssHII

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Order BssSαI

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Order BstAPI

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstXI

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Order BstYI

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Order BstZ17I-HF®

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Order Bsu36I

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Order BtgI

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Order BtgZI

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Order BtsCI

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Order BtsIMutI

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Order BtsαI

Restriction Endonucleases: C-G

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CviAII

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Order CviKI-1

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Order CviQI

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Order DdeI

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EaeI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order EciI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order FatI

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Order FauI

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Order Fnu4HI

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Order FokI

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Order FseI

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Order FspEI

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Order FspI

Restriction Endonucleases: H-M

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Order HaeII

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Order HaeIII

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Order HgaI

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Order HhaI

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Order HincII

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Order HindIII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaI

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Order HpaII

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Order HphI

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Order Hpy166II

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Order Hpy188III

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Order Hpy99I

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Order HpyAV

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Order HpyCH4III

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Order HpyCH4IV

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Order HpyCH4V

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Order I-CeuI

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Order I-SceI

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Order KasI

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Order KpnI

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Order KpnI-HF®

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Order LpnPI

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Order MboI

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Order MboII

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Order MfeI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MluI-HF®

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Order MlyI

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Order MmeI

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Order MnlI

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Order MscI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MspJI

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Order MwoI

Restriction Endonucleases: N-O

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Order NaeI

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Order NarI

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Order Nb.BbvCI

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Order Nb.BsmI

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Order Nb.BsrDI

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Order Nb.BssSI

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Order Nb.BtsI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NlaIV

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Order NmeAIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NruI-HF®

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Order NsiI

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Order NsiI-HF®

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Order NspI

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Order Nt.AlwI

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Order Nt.BbvCI

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Order Nt.BsmAI

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Order Nt.BspQI

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Order Nt.BstNBI

Restriction Endonucleases: P-R

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Order PacI

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Order PaeR7I

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Order PciI

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Order PflFI

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Order PflMI

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Order PI-PspI

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Order PI-SceI

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Order PleI

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Order PluTI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PsiI

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Order PspGI

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Order PspOMI

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Order PspXI

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Order PstI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order RsrII

Restriction Endonucleases: S

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Order SacI

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Order SacI-HF®

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Order SacII

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Order SalI

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Order SalI-HF®

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Order SapI

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Order Sau3AI

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Order Sau96I

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order ScrFI

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Order SexAI

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Order SfaNI

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Order SfcI

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Order SfiI

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Order SfoI

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Order SgrAI

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Order SmaI

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Order SmlI

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Order SnaBI

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Order SpeI

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Order SpeI-HF®

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Order SphI

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Order SphI-HF®

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Order SrfI

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Order SspI

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

Restriction Endonucleases: T-Z

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Order TaqαI

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Order TfiI

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Order TseI

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Order Tsp45I

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XcmI

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Order XhoI

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Order XmaI

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Order XmnI

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Order ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

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Order AatII

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Order AbaSI

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AflII

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Order AgeI-HF®

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Order AhdI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AvaI

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Order AvaII

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Order AvrII

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BbsI

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Order BbsI-HF®

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Order BbvI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmtI-HF®

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Order BpmI

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Order BpuEI

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Order BsaAI

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Order BsaHI

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Order BsaI-HF®

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BsgI

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Order BsiEI

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Order BsiWI

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Order BsiWI-HF®

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspEI

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Order BspHI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrGI

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Order BsrGI-HF®

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Order BsrI

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Order BssHII

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstXI

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Order BstYI

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Order BstZ17I-HF®

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Order Bsu36I

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Order BtgI

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Order BtsCI

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CviAII

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Order CviQI

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Order DdeI

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order Fnu4HI

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Order FseI

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Order FspEI

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Order FspI

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Order HaeIII

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Order HhaI

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Order HincII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaII

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Order HphI

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Order Hpy166II

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Order HpyAV

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Order HpyCH4IV

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Order HpyCH4V

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Order I-CeuI

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Order I-SceI

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Order KpnI

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Order KpnI-HF®

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Order LpnPI

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Order MboI

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Order McrBC

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MluI-HF®

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Order MlyI

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Order MmeI

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Order MnlI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MspJI

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Order MwoI

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Order Nb.BbvCI

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Order Nb.BsmI

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Order Nb.BsrDI

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Order Nb.BssSI

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Order Nb.BtsI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NruI-HF®

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Order NsiI

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Order NsiI-HF®

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Order NspI

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Order Nt.AlwI

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Order Nt.BbvCI

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Order Nt.BsmAI

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Order Nt.BspQI

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Order Nt.BstNBI

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Order PacI

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Order PaeR7I

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Order PflFI

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Order PflMI

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Order PI-PspI

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Order PI-SceI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PstI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order SacI

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Order SacI-HF®

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Order SacII

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Order SalI

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Order SalI-HF®

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Order SapI

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order SfiI

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Order SfoI

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Order SmaI

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Order SpeI

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Order SpeI-HF®

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Order SphI-HF®

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

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Order TaqαI

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Order TfiI

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Order TseI

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XhoI

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Order XmaI

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Order XmnI

Restriction Endonuclease Buffers & Diluents

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Order BSA, Molecular Biology Grade

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Order CutSmart® Buffer

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Order Diluent A

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Order Diluent B

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Order Diluent C

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Order NEBuffer™ 1

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Order NEBuffer™ 1.1

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Order NEBuffer™ 2

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Order NEBuffer™ 2.1

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Order NEBuffer™ 3

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Order NEBuffer™ 3.1

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Order NEBuffer™ 4

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Order NEBuffer™ EcoRI

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Order NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

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Order S-adenosylmethionine (SAM)

Restriction Endonucleases: A

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Order AatII

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Order AbaSI

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Order Acc65I

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Order AccI

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Order AciI

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Order AclI

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Order AcuI

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Order AfeI

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Order AflII

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Order AflIII

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Order AgeI

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Order AgeI-HF®

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Order AhdI

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Order AleI

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Order AluI

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Order AlwNI

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Order ApaI

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Order ApaLI

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Order ApeKI

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Order ApoI-HF®

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Order AscI

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Order AseI

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Order AsiSI

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Order AvaI

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Order AvaII

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Order AvrII

Restriction Endonucleases: B

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Order BaeGI

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Order BaeI

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Order BamHI

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Order BamHI-HF®

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Order BanI

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Order BanII

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Order BbsI

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Order BbsI-HF®

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Order BbvCI

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Order BbvI

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Order BccI

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Order BceAI

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Order BcgI

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Order BciVI

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Order BclI

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Order BclI-HF

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Order BcoDI

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Order BfaI

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Order BfuAI

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Order BglI

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Order BglII

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Order BlpI

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Order BmgBI

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Order BmrI

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Order BmtI

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Order BmtI-HF®

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Order BpmI

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Order Bpu10I

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Order BpuEI

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Order BsaAI

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Order BsaBI

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Order BsaHI

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Order BsaI

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Order BsaI-HF®

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Order BsaJI

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Order BsaWI

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Order BsaXI

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Order BseRI

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Order BseYI

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Order BsgI

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Order BsiEI

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Order BsiHKAI

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Order BsiWI

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Order BsiWI-HF®

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Order BslI

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Order BsmAI

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Order BsmBI

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Order BsmFI

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Order BsmI

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Order BsoBI

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Order Bsp1286I

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Order BspCNI

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Order BspDI

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Order BspEI

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Order BspHI

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Order BspMI

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Order BspQI

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Order BsrBI

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Order BsrDI

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Order BsrFαI

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Order BsrGI

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Order BsrGI-HF®

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Order BsrI

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Order BssHII

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Order BssSαI

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Order BstAPI

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Order BstBI

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Order BstEII

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Order BstEII-HF®

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Order BstNI

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Order BstUI

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Order BstXI

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Order BstYI

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Order BstZ17I-HF®

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Order Bsu36I

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Order BtgI

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Order BtgZI

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Order BtsCI

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Order BtsIMutI

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Order BtsαI

Restriction Endonucleases: C-G

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Order Cac8I

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Order ClaI

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Order CspCI

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Order CviAII

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Order CviKI-1

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Order CviQI

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Order DdeI

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Order DpnI

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Order DpnII

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Order DraI

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Order DraIII-HF®

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Order DrdI

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Order EaeI

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Order EagI

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Order EagI-HF®

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Order EarI

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Order EciI

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Order Eco53kI

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Order EcoNI

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Order EcoO109I

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Order EcoP15I

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Order EcoRI

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Order EcoRI-HF®

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Order EcoRV

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Order EcoRV-HF®

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Order FatI

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Order FauI

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Order Fnu4HI

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Order FokI

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Order FseI

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Order FspEI

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Order FspI

Restriction Endonucleases: H-M

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Order HaeII

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Order HaeIII

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Order HgaI

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Order HhaI

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Order HincII

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Order HindIII

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Order HindIII-HF®

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Order HinfI

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Order HinP1I

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Order HpaI

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Order HpaII

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Order HphI

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Order Hpy166II

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Order Hpy188III

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Order Hpy99I

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Order HpyAV

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Order HpyCH4III

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Order HpyCH4IV

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Order HpyCH4V

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Order I-CeuI

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Order I-SceI

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Order KasI

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Order KpnI

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Order KpnI-HF®

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Order LpnPI

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Order MboI

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Order MboII

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Order MfeI

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Order MfeI-HF®

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Order MluCI

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Order MluI

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Order MluI-HF®

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Order MlyI

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Order MmeI

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Order MnlI

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Order MscI

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Order MseI

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Order MslI

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Order MspA1I

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Order MspI

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Order MspJI

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Order MwoI

Restriction Endonucleases: N-O

Close

Order NaeI

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Order NarI

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Order Nb.BbvCI

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Order Nb.BsmI

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Order Nb.BsrDI

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Order Nb.BssSI

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Order Nb.BtsI

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Order NciI

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Order NcoI

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Order NcoI-HF®

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Order NdeI

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Order NgoMIV

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Order NheI

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Order NheI-HF®

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Order NlaIII

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Order NlaIV

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Order NmeAIII

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Order NotI

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Order NotI-HF®

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Order NruI

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Order NruI-HF®

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Order NsiI

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Order NsiI-HF®

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Order NspI

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Order Nt.AlwI

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Order Nt.BbvCI

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Order Nt.BsmAI

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Order Nt.BspQI

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Order Nt.BstNBI

Restriction Endonucleases: P-R

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Order PacI

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Order PaeR7I

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Order PciI

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Order PflFI

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Order PflMI

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Order PI-PspI

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Order PI-SceI

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Order PleI

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Order PluTI

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Order PmeI

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Order PpuMI

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Order PshAI

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Order PsiI

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Order PspGI

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Order PspOMI

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Order PspXI

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Order PstI

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Order PstI-HF®

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Order PvuI

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Order PvuI-HF®

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Order PvuII

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Order PvuII-HF®

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Order RsaI

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Order RsrII

Restriction Endonucleases: S

Close

Order SacI

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Order SacI-HF®

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Order SacII

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Order SalI

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Order SalI-HF®

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Order SapI

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Order Sau3AI

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Order Sau96I

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Order SbfI

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Order SbfI-HF®

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Order ScaI-HF®

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Order ScrFI

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Order SexAI

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Order SfaNI

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Order SfcI

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Order SfiI

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Order SfoI

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Order SgrAI

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Order SmaI

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Order SmlI

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Order SnaBI

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Order SpeI

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Order SpeI-HF®

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Order SphI

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Order SphI-HF®

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Order SrfI

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Order SspI

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Order SspI-HF®

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Order StuI

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Order StyD4I

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Order StyI

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Order StyI-HF®

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Order SwaI

Restriction Endonucleases: T-Z

Close

Order TaqαI

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Order TfiI

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Order TseI

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Order Tsp45I

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Order TspMI

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Order TspRI

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Order Tth111I

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Order XbaI

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Order XcmI

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Order XhoI

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Order XmaI

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Order XmnI

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Order ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

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Order AgeI-HF®

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Order ApoI-HF®

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Order BamHI-HF®

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Order BbsI-HF®

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Order BclI-HF

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Order BmtI-HF®

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Order BsaI-HF®

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  1. TypeII_RE_animation_Thumb_282x210

    Type II Restriction Enzymes

    Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work.

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    Type I Restriction Enzymes

    Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work.

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    Type III Restriction Enzymes

    Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes.

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    CutSmart Restriction Enzyme Buffer

    >210 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart® Buffer and why it matters to you.

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    Time-Saver Qualified Restriction Enzymes

    How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

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    Cloning With Restriction Enzymes

    Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.

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    Reduce Star Activity with High-Fidelity Restriction Enzymes

    NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

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    Standard Protocol for Restriction Enzyme Digests

    Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup.

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    What are Restriction Enzymes?

    Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology.

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    Why is My Restriction Enzyme Not Cutting DNA?

    Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction.

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    Restriction Enzyme Digest Problem: Too Many DNA Bands

    Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause.

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    Setting-up Restriction Enzyme Digests with RE-Mix Master Mixes

    RE-Mix® Restriction Enzyme Master Mixes offer simplified reaction setup. Learn more about digesting DNA with RE-Mix.

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    Restriction Enzyme Digest Protocol: Cutting Close to DNA End

    When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers.