Restriction Endonucleases

Time-Saver™ Qualified Restriction Enzymes

At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. This focus allows us to provide extremely pure enzymes at concentrations that deliver more flexibility to your experimental design.

Whether you are quickly screening large numbers of clones or setting up overnight digests, you will benefit from the high quality of our enzymes. Typically, a restriction digest involves the incubation of 1 µl of enzyme with 1 µg of purified DNA in a final volume of 50 µl for 1 hour. However, to speed up the screening process, choose a Time-Saver™ qualified enzyme for 5-15 minute digestion reactions. These enzymes will digest 1 µg of substrate DNA in 5-15 minutes using 1 µl of enzyme under recommended reaction conditions, and can also be used safely in overnight digestions. Unlike other suppliers, there is no special formulation, change in concentration or need to buy more expensive new lines of enzymes to achieve digestion in 5-15 minutes, nor do you have to worry if you incubate too long.

Power and Purity of Time-Saver Qualified Enzymes

HindIII-HF (NEB #R3104) is powerful enough to digest 1 µg of DNA in 5 minutes, but can also be used in overnight digests with no indication of nuclease contamination. Marker (M) is the 1 kb DNA Ladder (NEB #N3232)

In an effort to provide you with as much information as possible, NEB has tested all of its enzymes on unit assay substrate as well as plasmid substrate. We recommend that this be used as a guide as it is not definitive for all plasmids. Restriction enzymes can often show site preference, presumably determined by the sequence flanking the recognition site. In addition, supercoiled DNA may have varying rates of cleavage. Note that there are some enzymes that can digest DNA in 5-15 minutes, but cannot be incubated overnight. These are not Time-Saver qualified.

Since all of our enzymes are rigorously tested for nuclease contamination, you can also safely set up digests for long periods of time without sample degradation. Only NEB can offer enzymes with power and flexibility — the power to digest in 5-15 minutes and the flexibility to withstand overnight digestions with no loss of substrate.

  1. Time-Saver Qualified Restriction Enzymes

    How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.

  2. Time-Saver Protocol for Restriction Enzyme Digests

    Need a protocol to digest quickly and completely? Try this protocol for Time-Saver™ qualified enzymes from NEB.

  3. Reduce Star Activity with High-Fidelity Restriction Enzymes

    NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests.

  4. Discovering New Restriction Enzymes

    Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature.

  5. CutSmart Restriction Enzyme Buffer

    >210 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart® Buffer and why it matters to you.

    Other Tools & Resources


  • Restriction Endonucleases Technical Guide

    The Restriction Enzyme Technical Guide provides product information and technical reference charts on the wide range of restriction enzymes available from NEB.


  • Enzymes powerful enough to digest in 5-15 minutes
  • Flexible enough for overnight incubation
  • No special formulation
  • No added expense

Time-Saver Qualified Restriction Enzyme Selection Chart

Over 200 of NEB's restriction enzymes are Time-Saver Qualified. These enzymes will digest 1 µg of DNA in 15 minutes, yet are pure enough for overnight incubation, and are provided at no extra expense.