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Home Restriction Endonucleases Products BtgZI

BtgZI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCGATGNNNNNNNNNN^NNNN_

Isoschizomers | Single Letter Code | Pronunciation:

100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GCGATG(10/14)

Catalog #	Concentration	Size	List Price	Your Price
R0703S	5,000 units/ml	100 units	$75.00	Sign In Sign In
R0703L	5,000 units/ml	500 units	$308.00	Sign In Sign In

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Product Information

Product Source

An E.coli strain that carries the cloned BtgZI gene from Bacillus thermoglucosidasius (X. Pan)

This product is related to the following categories:: Restriction Endonucleases B

This product can be used in the following applications:: Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0703S -20

BtgZI R0703SVIAL -20 1 x 0.02 ml 5,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0703L -20

BtgZI R0703LVIAL -20 1 x 0.1 ml 5,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 60°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 60°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 10%
NEBuffer™ r2.1: 25%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Impaired

Activity at Temperature
@37°C: 50%
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. Cleavage of mammalian genomic DNA is impaired by CpG methylation.
2. BtgZI can remain bound to DNA after cutting and alter migration rate of DNA during electrophoresis. To disrupt binding, add SDS to a final concentration of 0.5% or purify DNA before electrophoresis.
3. Based on the stability of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
4. star activity may result from a glycerol concentration of >5%