Restriction Endonucleases - Survival in a Reaction

Restriction endonucleases vary with respect to their ability to maintain activity in a reaction over an extended period of time.

While most routine restriction digests are incubated for one hour or less, there are certain applications that require the addition of less than 1 unit/µg of DNA and increasing the reaction time beyond one hour. The table below can be used as a guide when low levels of enzyme and extended reaction times are needed.

For example, 1 unit of Aatll can be used to digest 8 µg of DNA in a 16 hour digest (+++).

Extended activity was determined by performing the restriction endonuclease unit assay, using a 16 hour incubation in place of the standard 1 hour digestion. After the 16 hour digestion, extended activity enzymes (+++) required only 0.13 units to completely digest 1 µg of DNA. Intermediate activity enzymes required either 0.25 (++) or 0.50 (+) units for complete digestion over this extended incubation time. Finally, enzymes marked (-) required 1.0 unit for complete digestion, the same amount of enzyme required for a 1 hour digestion. Substrate DNA is defined in the unit assay for a particular product, which is provided on the individual product page.

+++
++
+
Enzyme is active > 8 hours
Enzyme is active 4-8 hours
Enzyme is active 2-4 hours
Not benefit from digesting over 1 hour

EnzymeSuitability for Extended Digestions
AatII+++
AbaSI
Acc65I+
AccI+++
AciI-
AclI+
AcuI-
AfeI++
AflII+++
AflIII+++
AgeI+
AgeI-HF®++
AhdI+++
AleI++
AluI++
AlwI+
AlwNI+++
ApaI+++
ApaLI+++
ApeKI+++
EnzymeSuitability for Extended Digestions
ApoI+++
ApoI-HF+++
AscI+++
AseI+++
AsiSI+++
AvaI++
AvaII++
AvrII+++
EnzymeBack to topSuitability for Extended Digestions
BaeGI+
BaeI+
BamHI+
BamHI-HF®+
BanI+++
BanII+
BbsI++
BbsI-HF®-
BbvCI+++
BbvI-
BccI+
BceAI+++
EnzymeSuitability for Extended Digestions
BcgI+
BciVI-
BclI+
BcoDI+++
BfaI+
BfuAI++
BfuCI-
BglI+++
BglII++
BlpI+
BmgBI-
BmrI-
BmtI+
BmtI-HF®+++
BpmI-
Bpu10I+
BpuEI-
BsaAI++
BsaBI+
BsaHI+++
EnzymeSuitability for Extended Digestions
BsaI-
BsaI-HF®++
BsaJI+++
BsaWI+++
BsaXI++
BseRI+
BseYI++
BsgI+
BsiEI++
BsiHKAI-
BsiWI+++
BsiWI-HF®+++
BslI+++
BsmAI++
BsmBI+
BsmFI+++
BsmI+
BsoBI+++
Bsp1286I+
BspCNI-
EnzymeSuitability for Extended Digestions
BspDI++
BspEI+++
BspHI+++
BspMI++
BspQI-
BsrBI+
BsrDI+
BsrFαI+++
BsrFI+++
BsrGI+++
BsrGI-HF®
BsrI++
BssHII+
BssKI++
BssSαI+++
BssSI++
BstAPI++
BstBI+++
BstEII -
BstEII-HF®-
EnzymeSuitability for Extended Digestions
BstNI-
BstUI+++
BstXI+++
BstYI+++
BstZ17I-
BstZ17I-HF®++
Bsu36I+++
BtgI+
BtgZI-
BtsαI
BtsCI+
BtsI+
BtsIMutI+
EnzymeBack to topSuitability for Extended Digestions
Cac8I++
ClaI+
CspCI+
CviAII-
CviKI-1-
CviQI++
EnzymeBack to topSuitability for Extended Digestions
DdeI+++
EnzymeSuitability for Extended Digestions
DpnI+++
DpnII+++
DraI++
DraIII++
DraIII-HF®+++
DrdI+++
EnzymeBack to topSuitability for Extended Digestions
EaeI+++
EagI+++
EagI-HF®-
EarI+++
EciI-
Eco53kI++
EcoNI+++
EcoO109I+++
EcoP15I-
EcoRI+++
EcoRI-HF®+++
EcoRV+
EcoRV-HF®+
EnzymeBack to topSuitability for Extended Digestions
FatI+
EnzymeSuitability for Extended Digestions
FauI-
Fnu4HI++
FokI-
FseI-
FspEI+++
FspI+++
EnzymeBack to topSuitability for Extended Digestions
HaeII-
HaeIII++
HgaI-
HhaI++
HincII+++
HindIII+++
HindIII-HF®+++
HinfI++
HinP1I++
HpaI++
HpaII++
HphI++
Hpy166II+++
Hpy188I-
EnzymeSuitability for Extended Digestions
Hpy188III++
Hpy99I-
HpyAV-
HpyCH4III+++
HpyCH4IV++
HpyCH4V++
EnzymeBack to topSuitability for Extended Digestions
I-CeuI++
I-SceI++
EnzymeBack to topSuitability for Extended Digestions
KasI-
KpnI++
KpnI-HF®+
EnzymeBack to topSuitability for Extended Digestions
LpnPI-
EnzymeBack to topSuitability for Extended Digestions
MboI+
MboII-
MfeI++
MfeI-HF®++
MluCI-
MluI+++
MluI-HF®
MlyI-
EnzymeSuitability for Extended Digestions
MmeI-
MnlI++
MscI+
MseI+++
MslI-
MspA1I++
MspI+
MspJI+++
MwoI+++
EnzymeBack to topSuitability for Extended Digestions
NaeI+
NarI-
Nb.BbvCI+++
Nb.BsmI++
Nb.BsrDI++
Nb.BssSI+++
Nb.BtsI++
NciI+
NcoI++
NcoI-HF®++
NdeI+++
EnzymeSuitability for Extended Digestions
NgoMIV+++
NheI++
NheI-HF®+++
NlaIII+
NlaIV+
NmeAIII-
NotI++
NotI-HF®+++
NruI+++
NruI-HF®
NsiI++
NsiI-HF®
NspI++
Nt.AlwI+++
Nt.BbvCI+++
Nt.BsmAI+++
Nt.BspQI++
Nt.BstNBI+
Nt.CviPII-
EnzymeBack to topSuitability for Extended Digestions
PacI+++
EnzymeSuitability for Extended Digestions
PaeR7I+++
PciI++
PflFI+++
PflMI+
PhoI+++
PI-PspI+++
PI-SceI+++
PleI+
PluTI+
PmeI-
PmlI+
PpuMI+++
PshAI-
PsiI++
PspGI++
PspOMI+++
PspXI+++
PstI+
PstI-HF®+
PvuI+++
EnzymeSuitability for Extended Digestions
PvuI-HF®+++
PvuII+++
PvuII-HF®-
EnzymeBack to topSuitability for Extended Digestions
RsaI++
RsrII++
EnzymeBack to topSuitability for Extended Digestions
SacI+++
SacI-HF®+++
SacII+++
SalI+++
SalI-HF®++
SapI-
Sau3AI+
Sau96I++
SbfI+
SbfI-HF®-
ScaI++
ScaI-HF®++
ScrFI++
SexAI++
SfaNI+
EnzymeSuitability for Extended Digestions
SfcI-
SfiI++
SfoI-
SgrAI-
SmaI+++
SmlI++
SnaBI+
SpeI++
SpeI-HF®+
SphI+++
SphI-HF®-
SrfI+++
SspI++
SspI-HF®+
StuI++
StyD4I+++
StyI++
StyI-HF®++
SwaI++
EnzymeBack to topSuitability for Extended Digestions
TaqαI+
EnzymeSuitability for Extended Digestions
TfiI++
TliI++
TseI+
Tsp45I+
Tsp509I+++
TspMI+++
TspRI+++
Tth111I++
EnzymeBack to topSuitability for Extended Digestions
XbaI+++
XcmI+++
XhoI+++
XmaI+
XmnI++
EnzymeBack to topSuitability for Extended Digestions
ZraI+