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Home Restriction Endonucleases Products BsaXI

BsaXI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence _NNN^NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN^

Isoschizomers | Single Letter Code | Pronunciation:

100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Cut Site: (9/12)ACNNNNNCTCC(10/7)

Catalog #	Concentration	Size	List Price	Your Price
R0609S	2,000 units/ml	100 units	$75.00	Sign In Sign In
R0609L	2,000 units/ml	500 units	$308.00	Sign In Sign In

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Product Information

Product Source

Bacillus stearothermophilus 25B (Z. Chen)

This product is related to the following categories:: Restriction Endonucleases B,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0609S -20

BsaXI R0609SVIAL -20 1 x 0.05 ml 2,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0609L -20

BsaXI R0609LVIAL -20 1 x 0.25 ml 2,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent C
Storage Buffer

10 mM Tris-HCl
500 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.6 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. BsaXI cleaves DNA substrates twice to excise its recognition site generating a 27 base-pair fragment with 3-base 3' overhangs.
2. Addition of greater than 2 units of BsaXI in a 16 hour incubation is not recommended due to DNA binding.
3. May exhibit star activity in NEBuffer r1.1, and NEBuffer r2.1.
4. For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
5. Not sensitive to CpG, dcm, or dam methylation