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Home Restriction Endonucleases Products PvuII

PvuII

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence CAG_^CTG

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3151) supplied with rCutSmart^™ Buffer
Restriction Enzyme Cut Site: CAG/CTG

Catalog #	Concentration	Size	List Price	Your Price
R0151S	10,000 units/ml	5,000 units	$67.00	Sign In Sign In
R0151T	50,000 units/ml	5,000 units	$67.00	Sign In Sign In
R0151L	10,000 units/ml	25,000 units	$273.00	Sign In Sign In
R0151M	50,000 units/ml	25,000 units	$273.00	Sign In Sign In

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Product Information

PvuII has a High Fidelity version PvuII-HF^® (NEB #R3151).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

PvuII crystals (Ira Schildkraut and Joseph Bonventre, New England Biolabs)

Product Source

An E.coli strain that carries the PvuII gene from Proteus vulgaris (ATCC 13315).

This product is related to the following categories:: Restriction Endonucleases P R,; Time-Saver Qualified Restriction Enzymes Products

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0151S -20

PvuII R0151SVIAL -20 1 x 0.5 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

R0151T -20

PvuII R0151TVIAL -20 1 x 0.1 ml 50,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

R0151L -20

PvuII R0151LVIAL -20 2 x 1.25 ml 10,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

R0151M -20

PvuII R0151MVIAL -20 1 x 0.5 ml 50,000 units/ml

NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 37°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent B
Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Isoschizomers

PvuII-HF
Related Products
Companion Products
Materials Sold Separately
- NEBuffer™ r3.1
Product Notes
1. May exhibit star activity in rCutSmart Buffer.
2. PvuII does not cut CAGm4CTG, while cutting at CAGm5CTG occurs at a significantly reduced rate.
3. For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.