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PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.

PCR, Polymerases & Amplification Technologies

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Order Luna® Universal One-Step RT-qPCR Kit

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Order Luna® Universal Probe One-Step RT-qPCR Kit

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Order Luna® Universal Probe qPCR Master Mix

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Order Luna® Universal qPCR Master Mix

Q5® High-Fidelity DNA Polymerases

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Order NEBNext® High-Fidelity 2X PCR Master Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order Q5® High-Fidelity 2X Master Mix

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Order Q5® High-Fidelity DNA Polymerase

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Order Q5® High-Fidelity PCR Kit

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Q5® Hot Start High-Fidelity DNA Polymerase

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Order Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® DNA Polymerase

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start DNA Polymerase

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

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Order EpiMark® Hot Start Taq DNA Polymerase

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Order Hot Start Taq 2X Master Mix

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Order Hot Start Taq DNA Polymerase

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Order Multiplex PCR 5X Master Mix

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® DNA Polymerase

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start DNA Polymerase

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Order Quick-Load® Taq 2X Master Mix

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order Taq 2X Master Mix

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Order Taq 5X Master Mix

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Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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Order Taq DNA Polymerase with Standard Taq Buffer

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Order Taq DNA Polymerase with ThermoPol® Buffer

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Order Taq PCR Kit

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

LongAmp® DNA Polymerases

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Order LongAmp® Hot Start Taq 2X Master Mix

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Order LongAmp® Hot Start Taq DNA Polymerase

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Order LongAmp® Taq 2X Master Mix

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Order LongAmp® Taq DNA Polymerase

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Order LongAmp® Taq PCR Kit

Luna® qPCR & RT-qPCR Products

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Order Luna® Universal One-Step RT-qPCR Kit

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Order Luna® Universal Probe One-Step RT-qPCR Kit

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Order Luna® Universal Probe qPCR Master Mix

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Order Luna® Universal qPCR Master Mix

Other PCR Polymerases

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Order Deep Vent® (exo-) DNA Polymerase

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Order Deep Vent® DNA Polymerase

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Order Hemo KlenTaq®

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Order Vent® (exo-) DNA Polymerase

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Order Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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Order Phusion® GC Buffer Pack

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Order Phusion® HF Buffer Pack

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Order Phusion® High-Fidelity DNA Polymerase

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Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Order Phusion® Hot Start Flex 2X Master Mix

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Order Phusion® Hot Start Flex DNA Polymerase

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Order Phusion® High-Fidelity PCR Kit

Master Mixes

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Order Hot Start Taq 2X Master Mix

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Order LongAmp® Hot Start Taq 2X Master Mix

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Order LongAmp® Taq 2X Master Mix

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Order Multiplex PCR 5X Master Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Order Phusion® Hot Start Flex 2X Master Mix

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Order Q5® High-Fidelity 2X Master Mix

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Quick-Load® Taq 2X Master Mix

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Order Taq 2X Master Mix

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Order Taq 5X Master Mix

DNA Manipulation

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Order Bsu DNA Polymerase, Large Fragment

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Order DNA Polymerase I (E. coli)

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Order DNA Polymerase I, Large (Klenow) Fragment

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Order Klenow Fragment (3'→5' exo-)

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Order PreCR® Repair Mix

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Order Quick Blunting™ Kit

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Order Sulfolobus DNA Polymerase IV

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Order T4 DNA Polymerase

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Order T7 DNA Polymerase (unmodified)

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Order Terminal Transferase

Isothermal Amplification & Strand Displacement

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Order Bst 2.0 DNA Polymerase

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Order Bst 2.0 WarmStart® DNA Polymerase

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Order Bst 3.0 DNA Polymerase

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Order Bst DNA Polymerase, Full Length

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Order Bst DNA Polymerase, Large Fragment

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Order IsoAmp® II Universal tHDA Kit

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Order Isothermal Amplification Buffer II Pack

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Order Isothermal Amplification Buffer Pack

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Order Klenow Fragment (3'→5' exo-)

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Order phi29 DNA Polymerase

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Order WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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Order WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

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Order 3´-Biotin-GTP

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Order 3´-Desthiobiotin-GTP

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Order 5-methyl-dCTP

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Order 7-deaza-dGTP

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Order Acyclonucleotide Set

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Order Adenosine 5'-Triphosphate (ATP)

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Order dATP Solution

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Order Deoxynucleotide (dNTP) Solution Mix

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Order Deoxynucleotide (dNTP) Solution Set

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Order Ribonucleotide Solution Mix

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Order Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

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Order AMV Reverse Transcriptase

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Order DNA Polymerase I (E. coli)

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Order E. coli Poly(A) Polymerase

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Order M13KO7 Helper Phage

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Order M-MuLV Reverse Transcriptase

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order Oligo d(T)18 mRNA Primer

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Order Oligo d(T)23 VN

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Order OneTaq® One-Step RT-PCR Kit

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Order OneTaq® RT-PCR Kit

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Order Poly(U) Polymerase

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order Random Primer 9

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Order RNase H

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Order RNase Inhibitor, Murine

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Order SP6 RNA Polymerase

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Order T3 RNA Polymerase

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Order T4 RNA Ligase 2, truncated KQ

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Order WarmStart® RTx Reverse Transcriptase

RT-PCR

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Order AMV Reverse Transcriptase

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Order M-MuLV Reverse Transcriptase

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Order Oligo d(T)18 mRNA Primer

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Order Oligo d(T)23 VN

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Order OneTaq® One-Step RT-PCR Kit

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Order OneTaq® RT-PCR Kit

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order Random Primer 9

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Order T4 RNA Ligase 2, truncated KQ

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Order WarmStart® RTx Reverse Transcriptase

Reaction Buffers

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Order Isothermal Amplification Buffer II Pack

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Order Isothermal Amplification Buffer Pack

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Order Magnesium Chloride (MgCl2) Solution

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Order Magnesium Sulfate (MgSO4) Solution

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Order Phusion® GC Buffer Pack

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Order Phusion® HF Buffer Pack

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Order Q5® Reaction Buffer Pack

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

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Order β-Nicotinamide adenine dinucleotide (NAD+)

Q5® High-Fidelity DNA Polymerases

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Order NEBNext® High-Fidelity 2X PCR Master Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order NEBNext® Ultra™ II Q5® Master Mix

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Order Q5® High-Fidelity 2X Master Mix

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Order Q5® High-Fidelity DNA Polymerase

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Order Q5® High-Fidelity PCR Kit

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Q5® Hot Start High-Fidelity DNA Polymerase

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Order Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® DNA Polymerase

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start DNA Polymerase

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

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Order EpiMark® Hot Start Taq DNA Polymerase

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Order Hot Start Taq 2X Master Mix

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Order Hot Start Taq DNA Polymerase

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Order Multiplex PCR 5X Master Mix

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® DNA Polymerase

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start DNA Polymerase

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Order Quick-Load® Taq 2X Master Mix

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order Taq 2X Master Mix

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Order Taq 5X Master Mix

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Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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Order Taq DNA Polymerase with Standard Taq Buffer

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Order Taq DNA Polymerase with ThermoPol® Buffer

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Order Taq PCR Kit

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

LongAmp® DNA Polymerases

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Order LongAmp® Hot Start Taq 2X Master Mix

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Order LongAmp® Hot Start Taq DNA Polymerase

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Order LongAmp® Taq 2X Master Mix

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Order LongAmp® Taq DNA Polymerase

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Order LongAmp® Taq PCR Kit

Luna® qPCR & RT-qPCR Products

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Order Luna® Universal One-Step RT-qPCR Kit

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Order Luna® Universal Probe One-Step RT-qPCR Kit

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Order Luna® Universal Probe qPCR Master Mix

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Order Luna® Universal qPCR Master Mix

Other PCR Polymerases

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Order Deep Vent® (exo-) DNA Polymerase

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Order Deep Vent® DNA Polymerase

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Order Hemo KlenTaq®

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Order Vent® (exo-) DNA Polymerase

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Order Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

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Order Phusion® GC Buffer Pack

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Order Phusion® HF Buffer Pack

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Order Phusion® High-Fidelity DNA Polymerase

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Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Order Phusion® Hot Start Flex 2X Master Mix

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Order Phusion® Hot Start Flex DNA Polymerase

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Order Phusion® High-Fidelity PCR Kit

Master Mixes

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Order Hot Start Taq 2X Master Mix

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Order LongAmp® Hot Start Taq 2X Master Mix

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Order LongAmp® Taq 2X Master Mix

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Order Multiplex PCR 5X Master Mix

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Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

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Order OneTaq® 2X Master Mix with GC Buffer

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Order OneTaq® 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with GC Buffer

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Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

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Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

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Order Phusion® Hot Start Flex 2X Master Mix

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Order Q5® High-Fidelity 2X Master Mix

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Order Q5® Hot Start High-Fidelity 2X Master Mix

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Order Quick-Load® Taq 2X Master Mix

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Order Taq 2X Master Mix

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Order Taq 5X Master Mix

DNA Manipulation

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Order Bsu DNA Polymerase, Large Fragment

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Order DNA Polymerase I (E. coli)

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Order DNA Polymerase I, Large (Klenow) Fragment

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Order Klenow Fragment (3'→5' exo-)

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Order PreCR® Repair Mix

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Order Quick Blunting™ Kit

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Order Sulfolobus DNA Polymerase IV

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Order T4 DNA Polymerase

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Order T7 DNA Polymerase (unmodified)

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Order Terminal Transferase

Isothermal Amplification & Strand Displacement

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Order Bst 2.0 DNA Polymerase

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Order Bst 2.0 WarmStart® DNA Polymerase

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Order Bst 3.0 DNA Polymerase

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Order Bst DNA Polymerase, Full Length

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Order Bst DNA Polymerase, Large Fragment

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Order IsoAmp® II Universal tHDA Kit

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Order Isothermal Amplification Buffer II Pack

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Order Isothermal Amplification Buffer Pack

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Order Klenow Fragment (3'→5' exo-)

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Order phi29 DNA Polymerase

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Order WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA)

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Order WarmStart® LAMP Kit (DNA & RNA)

Nucleotide Solutions

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Order 3´-Biotin-GTP

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Order 3´-Desthiobiotin-GTP

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Order 5-methyl-dCTP

Close

Order 7-deaza-dGTP

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Order Acyclonucleotide Set

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Order Adenosine 5'-Triphosphate (ATP)

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Order dATP Solution

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Order Deoxynucleotide (dNTP) Solution Mix

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Order Deoxynucleotide (dNTP) Solution Set

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Order Ribonucleotide Solution Mix

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Order Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

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Order AMV Reverse Transcriptase

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Order DNA Polymerase I (E. coli)

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Order E. coli Poly(A) Polymerase

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Order M13KO7 Helper Phage

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Order M-MuLV Reverse Transcriptase

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Order NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module

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Order Oligo d(T)18 mRNA Primer

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Order Oligo d(T)23 VN

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Order OneTaq® One-Step RT-PCR Kit

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Order OneTaq® RT-PCR Kit

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Order Poly(U) Polymerase

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order Random Primer 9

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Order RNase H

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Order RNase Inhibitor, Murine

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Order SP6 RNA Polymerase

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Order T3 RNA Polymerase

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Order T4 RNA Ligase 2, truncated KQ

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Order WarmStart® RTx Reverse Transcriptase

RT-PCR

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Order AMV Reverse Transcriptase

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Order M-MuLV Reverse Transcriptase

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Order Oligo d(T)18 mRNA Primer

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Order Oligo d(T)23 VN

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Order OneTaq® One-Step RT-PCR Kit

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Order OneTaq® RT-PCR Kit

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Order ProtoScript® First Strand cDNA Synthesis Kit

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Order ProtoScript® II First Strand cDNA Synthesis Kit

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Order ProtoScript® II Reverse Transcriptase

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Order Random Primer 9

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Order T4 RNA Ligase 2, truncated KQ

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Order WarmStart® RTx Reverse Transcriptase

Reaction Buffers

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Order Isothermal Amplification Buffer II Pack

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Order Isothermal Amplification Buffer Pack

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Order Magnesium Chloride (MgCl2) Solution

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Order Magnesium Sulfate (MgSO4) Solution

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Order Phusion® GC Buffer Pack

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Order Phusion® HF Buffer Pack

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Order Q5® Reaction Buffer Pack

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Order Standard Taq (Mg-free) Reaction Buffer Pack

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Order Standard Taq Reaction Buffer Pack

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Order ThermoPol® II (Mg-free) Reaction Buffer Pack

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Order ThermoPol® Reaction Buffer Pack

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Order β-Nicotinamide adenine dinucleotide (NAD+)