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PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.

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PCR, qPCR & Amplification Technologies
Q5® High-Fidelity DNA Polymerases
OneTaq® DNA Polymerases
Taq DNA Polymerases
LongAmp® DNA Polymerases
Luna® qPCR & RT-qPCR Products
Other PCR Polymerases
Phusion® High-Fidelity DNA Polymerases
Master Mixes
DNA Manipulation
Isothermal Amplification & Strand Displacement
Nucleotide Solutions
cDNA Synthesis & Reverse Transcriptases
RT-PCR
Reaction Buffers

Luna^® Cell Ready Lysis Module

Luna^® Cell Ready One-Step RT-qPCR Kit

Luna^® Cell Ready Probe One-Step RT-qPCR Kit

Luna^® Probe One-Step RT-qPCR Kit (No ROX)

Luna^® SARS-CoV-2 RT-qPCR Multiplex Assay Kit

Luna^® Universal One-Step RT-qPCR Kit

Luna^® Universal Probe One-Step RT-qPCR Kit

Luna^® Universal Probe qPCR Master Mix

Luna^® Universal qPCR Master Mix

OneTaq^® Quick-Load^® DNA Polymerase

Q5U^® Hot Start High-Fidelity DNA Polymerase

SARS-CoV-2 Positive Control (N gene)

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit

Template Switching RT Enzyme Mix

WarmStart^® Colorimetric LAMP 2X Master Mix with UDG

Q5® High-Fidelity DNA Polymerases

NEBNext^® High-Fidelity 2X PCR Master Mix

NEBNext^® Q5^® Hot Start HiFi PCR Master Mix

NEBNext^® Ultra™ II Q5^® Master Mix

Q5^® High-Fidelity 2X Master Mix

Q5^® High-Fidelity DNA Polymerase

Q5^® Hot Start High-Fidelity 2X Master Mix

Q5^® Hot Start High-Fidelity DNA Polymerase

Q5U^® Hot Start High-Fidelity DNA Polymerase

OneTaq® DNA Polymerases

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Quick-Load^® DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

EpiMark^® Hot Start Taq DNA Polymerase

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Quick-Load^® Taq 2X Master Mix

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol^® Buffer

ThermoPol^® Reaction Buffer Pack

LongAmp® DNA Polymerases

LongAmp^® Hot Start Taq 2X Master Mix

LongAmp^® Hot Start Taq DNA Polymerase

Luna® qPCR & RT-qPCR Products

Luna^® Cell Ready Lysis Module

Luna^® Cell Ready One-Step RT-qPCR Kit

Luna^® Cell Ready Probe One-Step RT-qPCR Kit

Luna^® Probe One-Step RT-qPCR 4X Mix with UDG

Luna^® Probe One-Step RT-qPCR Kit (No ROX)

Luna^® SARS-CoV-2 RT-qPCR Multiplex Assay Kit

Luna^® Universal One-Step RT-qPCR Kit

Luna^® Universal Probe One-Step RT-qPCR Kit

Luna^® Universal Probe qPCR Master Mix

Luna^® Universal qPCR Master Mix

LunaScript^® RT Master Mix Kit (Primer-free)

Other PCR Polymerases

Deep Vent^® (exo-) DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Phusion^® High-Fidelity DNA Polymerase

Phusion^® High-Fidelity PCR Master Mix with GC Buffer

Phusion^® High-Fidelity PCR Master Mix with HF Buffer

Phusion^® Hot Start Flex 2X Master Mix

Phusion^® Hot Start Flex DNA Polymerase

Phusion^® High-Fidelity PCR Kit

Master Mixes

LongAmp^® Hot Start Taq 2X Master Mix

NEBNext^® Q5^® Hot Start HiFi PCR Master Mix

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Phusion^® High-Fidelity PCR Master Mix with GC Buffer

Phusion^® High-Fidelity PCR Master Mix with HF Buffer

Phusion^® Hot Start Flex 2X Master Mix

Q5^® High-Fidelity 2X Master Mix

Q5^® Hot Start High-Fidelity 2X Master Mix

Quick-Load^® Taq 2X Master Mix

WarmStart^® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

DNA Manipulation

Bsu DNA Polymerase, Large Fragment

DNA Polymerase I, Large (Klenow) Fragment

T7 DNA Polymerase (unmodified)

Isothermal Amplification & Strand Displacement

Bst 2.0 WarmStart^® DNA Polymerase

Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Large Fragment

IsoAmp^® II Universal tHDA Kit

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

SARS-CoV-2 Positive Control (N gene)

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit

WarmStart^® Colorimetric LAMP 2X Master Mix (DNA & RNA)

WarmStart^® Colorimetric LAMP 2X Master Mix with UDG

WarmStart^® LAMP Kit (DNA & RNA)

WarmStart^® Fluorescent LAMP/RT-LAMP Kit (with UDG)

WarmStart^® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

Nucleotide Solutions

Adenosine 5'-Triphosphate (ATP)

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

cDNA Synthesis & Reverse Transcriptases

LunaScript^® RT Master Mix Kit (Primer-free)

LunaScript^® Multiplex One-Step RT-PCR Kit

NEBNext^® Ultra™ II Non-Directional RNA Second Strand Synthesis Module

ProtoScript^® First Strand cDNA Synthesis Kit

ProtoScript^® II First Strand cDNA Synthesis Kit

ProtoScript^® II Reverse Transcriptase

Template Switching RT Enzyme Mix

WarmStart^® RTx Reverse Transcriptase

RT-PCR

LunaScript^® RT Master Mix Kit (Primer-free)

LunaScript^® Multiplex One-Step RT-PCR Kit

ProtoScript^® First Strand cDNA Synthesis Kit

ProtoScript^® II First Strand cDNA Synthesis Kit

ProtoScript^® II Reverse Transcriptase

WarmStart^® RTx Reverse Transcriptase

Reaction Buffers

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

Magnesium Chloride (MgCl₂) Solution

Magnesium Sulfate (MgSO₄) Solution

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

ThermoPol^® Reaction Buffer Pack

β-Nicotinamide adenine dinucleotide (NAD⁺)

Q5® High-Fidelity DNA Polymerases

NEBNext^® High-Fidelity 2X PCR Master Mix

NEBNext^® Q5^® Hot Start HiFi PCR Master Mix

NEBNext^® Ultra™ II Q5^® Master Mix

Q5^® High-Fidelity 2X Master Mix

Q5^® High-Fidelity DNA Polymerase

Q5^® Hot Start High-Fidelity 2X Master Mix

Q5^® Hot Start High-Fidelity DNA Polymerase

Q5U^® Hot Start High-Fidelity DNA Polymerase

OneTaq® DNA Polymerases

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Quick-Load^® DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

EpiMark^® Hot Start Taq DNA Polymerase

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start DNA Polymerase

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Quick-Load^® Taq 2X Master Mix

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with ThermoPol^® Buffer

ThermoPol^® Reaction Buffer Pack

LongAmp® DNA Polymerases

LongAmp^® Hot Start Taq 2X Master Mix

LongAmp^® Hot Start Taq DNA Polymerase

Luna^® Cell Ready Lysis Module

Luna^® Cell Ready One-Step RT-qPCR Kit

Luna^® Cell Ready Probe One-Step RT-qPCR Kit

Luna^® Probe One-Step RT-qPCR 4X Mix with UDG

Luna^® Probe One-Step RT-qPCR Kit (No ROX)

Luna^® SARS-CoV-2 RT-qPCR Multiplex Assay Kit

Luna^® Universal One-Step RT-qPCR Kit

Luna^® Universal Probe One-Step RT-qPCR Kit

Luna^® Universal Probe qPCR Master Mix

Luna^® Universal qPCR Master Mix

LunaScript^® RT Master Mix Kit (Primer-free)

Deep Vent^® (exo-) DNA Polymerase

Phusion^® High-Fidelity DNA Polymerase

Phusion^® High-Fidelity PCR Master Mix with GC Buffer

Phusion^® High-Fidelity PCR Master Mix with HF Buffer

Phusion^® Hot Start Flex 2X Master Mix

Phusion^® Hot Start Flex DNA Polymerase

Phusion^® High-Fidelity PCR Kit

Master Mixes

LongAmp^® Hot Start Taq 2X Master Mix

NEBNext^® Q5^® Hot Start HiFi PCR Master Mix

OneTaq^® 2X Master Mix with Standard Buffer

OneTaq^® Hot Start 2X Master Mix with GC Buffer

OneTaq^® Hot Start 2X Master Mix with Standard Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with GC Buffer

OneTaq^® Hot Start Quick-Load^® 2X Master Mix with Standard Buffer

OneTaq^® Quick-Load^® 2X Master Mix with Standard Buffer

Phusion^® High-Fidelity PCR Master Mix with GC Buffer

Phusion^® High-Fidelity PCR Master Mix with HF Buffer

Phusion^® Hot Start Flex 2X Master Mix

Q5^® High-Fidelity 2X Master Mix

Q5^® Hot Start High-Fidelity 2X Master Mix

Quick-Load^® Taq 2X Master Mix

WarmStart^® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

DNA Manipulation

Bsu DNA Polymerase, Large Fragment

DNA Polymerase I, Large (Klenow) Fragment

T7 DNA Polymerase (unmodified)

Terminal Transferase

Isothermal Amplification & Strand Displacement

Bst 2.0 WarmStart^® DNA Polymerase

Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Large Fragment

IsoAmp^® II Universal tHDA Kit

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

SARS-CoV-2 Positive Control (N gene)

SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit

WarmStart^® Colorimetric LAMP 2X Master Mix (DNA & RNA)

WarmStart^® Colorimetric LAMP 2X Master Mix with UDG

WarmStart^® LAMP Kit (DNA & RNA)

WarmStart^® Fluorescent LAMP/RT-LAMP Kit (with UDG)

WarmStart^® Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

Nucleotide Solutions

Adenosine 5'-Triphosphate (ATP)

Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Set

LunaScript^® RT Master Mix Kit (Primer-free)

LunaScript^® Multiplex One-Step RT-PCR Kit

NEBNext^® Ultra™ II Non-Directional RNA Second Strand Synthesis Module

ProtoScript^® First Strand cDNA Synthesis Kit

ProtoScript^® II First Strand cDNA Synthesis Kit

ProtoScript^® II Reverse Transcriptase

Template Switching RT Enzyme Mix

WarmStart^® RTx Reverse Transcriptase

RT-PCR

LunaScript^® RT Master Mix Kit (Primer-free)

LunaScript^® Multiplex One-Step RT-PCR Kit

ProtoScript^® First Strand cDNA Synthesis Kit

ProtoScript^® II First Strand cDNA Synthesis Kit

ProtoScript^® II Reverse Transcriptase

WarmStart^® RTx Reverse Transcriptase

Reaction Buffers

Isothermal Amplification Buffer II Pack

Isothermal Amplification Buffer Pack

Magnesium Chloride (MgCl₂) Solution

Magnesium Sulfate (MgSO₄) Solution

Standard Taq (Mg-free) Reaction Buffer Pack

Standard Taq Reaction Buffer Pack

ThermoPol^® Reaction Buffer Pack

β-Nicotinamide adenine dinucleotide (NAD⁺)

Related Technical Resources

TmCalculator
PCR Selector
PCR Troubleshooting Guide
PCR Brochure

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