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PCR, qPCR & Amplification Technologies

NEB offers a wide range of DNA polymerases, and through our commitment to research, ensures the development of innovative, high quality tools for DNA amplification, qPCR and PCR.

PCR, Polymerases & Amplification Technologies
Q5® High-Fidelity DNA Polymerases
OneTaq® DNA Polymerases
Taq DNA Polymerases
LongAmp® DNA Polymerases
Luna® qPCR & RT-qPCR Products
Other PCR Polymerases
Phusion® High-Fidelity DNA Polymerases
Master Mixes
DNA Manipulation
Isothermal Amplification & Strand Displacement
Nucleotide Solutions
cDNA Synthesis & Reverse Transcriptases
RT-PCR
Reaction Buffers

PCR, Polymerases & Amplification Technologies

Order Luna® Universal One-Step RT-qPCR Kit

Order Luna® Universal Probe One-Step RT-qPCR Kit

Order Luna® Universal Probe qPCR Master Mix

Order Luna® Universal qPCR Master Mix

Order OneTaq^® Quick-Load^® DNA Polymerase

Q5® High-Fidelity DNA Polymerases

Order NEBNext® High-Fidelity 2X PCR Master Mix

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Order NEBNext® Ultra™ II Q5® Master Mix

Order Q5® High-Fidelity 2X Master Mix

Order Q5® High-Fidelity DNA Polymerase

Order Q5® High-Fidelity PCR Kit

Order Q5® Hot Start High-Fidelity 2X Master Mix

Order Q5® Hot Start High-Fidelity DNA Polymerase

Order Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq^® Quick-Load^® DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

Order EpiMark^® Hot Start Taq DNA Polymerase

Order Hot Start Taq 2X Master Mix

Order Hot Start Taq DNA Polymerase

Order Multiplex PCR 5X Master Mix

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Order Quick-Load® Taq 2X Master Mix

Order Standard Taq (Mg-free) Reaction Buffer Pack

Order Standard Taq Reaction Buffer Pack

Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Order Taq DNA Polymerase with Standard Taq Buffer

Order Taq DNA Polymerase with ThermoPol® Buffer

Order ThermoPol® II (Mg-free) Reaction Buffer Pack

Order ThermoPol^®Reaction Buffer Pack

LongAmp® DNA Polymerases

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Hot Start Taq DNA Polymerase

Order LongAmp^® Taq 2X Master Mix

Order LongAmp^® Taq DNA Polymerase

Luna® qPCR & RT-qPCR Products

Order Luna® Universal One-Step RT-qPCR Kit

Order Luna® Universal Probe One-Step RT-qPCR Kit

Order Luna® Universal Probe qPCR Master Mix

Order Luna® Universal qPCR Master Mix

Order LunaScript^™ RT SuperMix Kit

Other PCR Polymerases

Order Deep Vent^® (exo-) DNA Polymerase

Order Deep Vent^® DNA Polymerase

Order Vent^® (exo-) DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Order Phusion® High-Fidelity DNA Polymerase

Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

Order Phusion® Hot Start Flex 2X Master Mix

Order Phusion® Hot Start Flex DNA Polymerase

Order Phusion® High-Fidelity PCR Kit

Master Mixes

Order Hot Start Taq 2X Master Mix

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Taq 2X Master Mix

Order Multiplex PCR 5X Master Mix

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

Order Phusion® Hot Start Flex 2X Master Mix

Order Q5® High-Fidelity 2X Master Mix

Order Q5® Hot Start High-Fidelity 2X Master Mix

Order Quick-Load® Taq 2X Master Mix

DNA Manipulation

Order Bsu DNA Polymerase, Large Fragment

Order DNA Polymerase I (E. coli)

Order DNA Polymerase I, Large (Klenow) Fragment

Order Klenow Fragment (3'→5' exo-)

Order Sulfolobus DNA Polymerase IV

Order T7 DNA Polymerase (unmodified)

Isothermal Amplification & Strand Displacement

Order Bst 2.0 WarmStart^® DNA Polymerase

Order Bst DNA Polymerase, Full Length

Order Bst DNA Polymerase, Large Fragment

Order IsoAmp® II Universal tHDA Kit

Order Isothermal Amplification Buffer II Pack

Order Isothermal Amplification Buffer Pack

Order Klenow Fragment (3'→5' exo-)

Order WarmStart^® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Order WarmStart^® LAMP Kit (DNA & RNA)

Nucleotide Solutions

Order Adenosine 5'-Triphosphate (ATP)

Order Deoxynucleotide (dNTP) Solution Mix

Order Deoxynucleotide (dNTP) Solution Set

Order Ribonucleotide Solution Mix

Order Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

Order AMV Reverse Transcriptase

Order DNA Polymerase I (E. coli)

Order E. coli Poly(A) Polymerase

Order M-MuLV Reverse Transcriptase

Order NEBNext^® Ultra II Non-Directional RNA Second Strand Synthesis Module

Order Oligo d(T)₁₈ mRNA Primer

Order OneTaq® One-Step RT-PCR Kit

Order ProtoScript® First Strand cDNA Synthesis Kit

Order ProtoScript® II First Strand cDNA Synthesis Kit

Order ProtoScript® II Reverse Transcriptase

Order T4 RNA Ligase 2, truncated KQ

Order WarmStart® RTx Reverse Transcriptase

RT-PCR

Order AMV Reverse Transcriptase

Order M-MuLV Reverse Transcriptase

Order Oligo d(T)₁₈ mRNA Primer

Order OneTaq® One-Step RT-PCR Kit

Order ProtoScript® First Strand cDNA Synthesis Kit

Order ProtoScript® II First Strand cDNA Synthesis Kit

Order ProtoScript® II Reverse Transcriptase

Order T4 RNA Ligase 2, truncated KQ

Order WarmStart® RTx Reverse Transcriptase

Reaction Buffers

Order Isothermal Amplification Buffer II Pack

Order Isothermal Amplification Buffer Pack

Order Magnesium Chloride (MgCl₂) Solution

Order Magnesium Sulfate (MgSO₄) Solution

Order Q5® Reaction Buffer Pack

Order Standard Taq (Mg-free) Reaction Buffer Pack

Order Standard Taq Reaction Buffer Pack

Order ThermoPol® II (Mg-free) Reaction Buffer Pack

Order ThermoPol^®Reaction Buffer Pack

Order β-Nicotinamide adenine dinucleotide (NAD⁺)

Q5® High-Fidelity DNA Polymerases

Order NEBNext® High-Fidelity 2X PCR Master Mix

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Order NEBNext® Ultra™ II Q5® Master Mix

Order Q5® High-Fidelity 2X Master Mix

Order Q5® High-Fidelity DNA Polymerase

Order Q5® High-Fidelity PCR Kit

Order Q5® Hot Start High-Fidelity 2X Master Mix

Order Q5® Hot Start High-Fidelity DNA Polymerase

Order Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq^® Quick-Load^® DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

Order EpiMark^® Hot Start Taq DNA Polymerase

Order Hot Start Taq 2X Master Mix

Order Hot Start Taq DNA Polymerase

Order Multiplex PCR 5X Master Mix

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Order Quick-Load® Taq 2X Master Mix

Order Standard Taq (Mg-free) Reaction Buffer Pack

Order Standard Taq Reaction Buffer Pack

Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Order Taq DNA Polymerase with Standard Taq Buffer

Order Taq DNA Polymerase with ThermoPol® Buffer

Order ThermoPol® II (Mg-free) Reaction Buffer Pack

Order ThermoPol^®Reaction Buffer Pack

LongAmp® DNA Polymerases

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Hot Start Taq DNA Polymerase

Order LongAmp^® Taq 2X Master Mix

Order LongAmp^® Taq DNA Polymerase

Luna® qPCR & RT-qPCR Products

Order Luna® Universal One-Step RT-qPCR Kit

Order Luna® Universal Probe One-Step RT-qPCR Kit

Order Luna® Universal Probe qPCR Master Mix

Order Luna® Universal qPCR Master Mix

Order LunaScript^™ RT SuperMix Kit

Other PCR Polymerases

Order Deep Vent^® (exo-) DNA Polymerase

Order Deep Vent^® DNA Polymerase

Order Vent^® (exo-) DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Order Phusion® High-Fidelity DNA Polymerase

Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

Order Phusion® Hot Start Flex 2X Master Mix

Order Phusion® Hot Start Flex DNA Polymerase

Order Phusion® High-Fidelity PCR Kit

Master Mixes

Order Hot Start Taq 2X Master Mix

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Taq 2X Master Mix

Order Multiplex PCR 5X Master Mix

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

Order Phusion® Hot Start Flex 2X Master Mix

Order Q5® High-Fidelity 2X Master Mix

Order Q5® Hot Start High-Fidelity 2X Master Mix

Order Quick-Load® Taq 2X Master Mix

DNA Manipulation

Order Bsu DNA Polymerase, Large Fragment

Order DNA Polymerase I (E. coli)

Order DNA Polymerase I, Large (Klenow) Fragment

Order Klenow Fragment (3'→5' exo-)

Order Sulfolobus DNA Polymerase IV

Order T7 DNA Polymerase (unmodified)

Isothermal Amplification & Strand Displacement

Order Bst 2.0 WarmStart^® DNA Polymerase

Order Bst DNA Polymerase, Full Length

Order Bst DNA Polymerase, Large Fragment

Order IsoAmp® II Universal tHDA Kit

Order Isothermal Amplification Buffer II Pack

Order Isothermal Amplification Buffer Pack

Order Klenow Fragment (3'→5' exo-)

Order WarmStart^® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Order WarmStart^® LAMP Kit (DNA & RNA)

Nucleotide Solutions

Order Adenosine 5'-Triphosphate (ATP)

Order Deoxynucleotide (dNTP) Solution Mix

Order Deoxynucleotide (dNTP) Solution Set

Order Ribonucleotide Solution Mix

Order Ribonucleotide Solution Set

cDNA Synthesis & Reverse Transcriptases

Order AMV Reverse Transcriptase

Order DNA Polymerase I (E. coli)

Order E. coli Poly(A) Polymerase

Order M-MuLV Reverse Transcriptase

Order NEBNext^® Ultra II Non-Directional RNA Second Strand Synthesis Module

Order Oligo d(T)₁₈ mRNA Primer

Order OneTaq® One-Step RT-PCR Kit

Order ProtoScript® First Strand cDNA Synthesis Kit

Order ProtoScript® II First Strand cDNA Synthesis Kit

Order ProtoScript® II Reverse Transcriptase

Order T4 RNA Ligase 2, truncated KQ

Order WarmStart® RTx Reverse Transcriptase

RT-PCR

Order AMV Reverse Transcriptase

Order M-MuLV Reverse Transcriptase

Order Oligo d(T)₁₈ mRNA Primer

Order OneTaq® One-Step RT-PCR Kit

Order ProtoScript® First Strand cDNA Synthesis Kit

Order ProtoScript® II First Strand cDNA Synthesis Kit

Order ProtoScript® II Reverse Transcriptase

Order T4 RNA Ligase 2, truncated KQ

Order WarmStart® RTx Reverse Transcriptase

Reaction Buffers

Order Isothermal Amplification Buffer II Pack

Order Isothermal Amplification Buffer Pack

Order Magnesium Chloride (MgCl₂) Solution

Order Magnesium Sulfate (MgSO₄) Solution

Order Q5® Reaction Buffer Pack

Order Standard Taq (Mg-free) Reaction Buffer Pack

Order Standard Taq Reaction Buffer Pack

Order ThermoPol® II (Mg-free) Reaction Buffer Pack

Order ThermoPol^®Reaction Buffer Pack

Order β-Nicotinamide adenine dinucleotide (NAD⁺)

New Products

OneTaq^® Quick-Load^® DNA Polymerase

Related Technical Resources

PCR Selection Tool
PCR Troubleshooting Guide
PCR Brochure

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PCR, Polymerases & Amplification Technologies

Order Luna® Universal One-Step RT-qPCR Kit

Order Luna® Universal Probe One-Step RT-qPCR Kit

Order Luna® Universal Probe qPCR Master Mix

Order Luna® Universal qPCR Master Mix

Order OneTaq® Quick-Load® DNA Polymerase

Q5® High-Fidelity DNA Polymerases

Order NEBNext® High-Fidelity 2X PCR Master Mix

Order NEBNext® Q5® Hot Start HiFi PCR Master Mix

Order NEBNext® Ultra™ II Q5® Master Mix

Order Q5® High-Fidelity 2X Master Mix

Order Q5® High-Fidelity DNA Polymerase

Order Q5® High-Fidelity PCR Kit

Order Q5® Hot Start High-Fidelity 2X Master Mix

Order Q5® Hot Start High-Fidelity DNA Polymerase

Order Q5® Reaction Buffer Pack

OneTaq® DNA Polymerases

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® DNA Polymerase

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Quick-Load® DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Taq DNA Polymerases

Order EpiMark® Hot Start Taq DNA Polymerase

Order Hot Start Taq 2X Master Mix

Order Hot Start Taq DNA Polymerase

Order Multiplex PCR 5X Master Mix

Order OneTaq® 2X Master Mix with Standard Buffer

Order OneTaq® DNA Polymerase

Order OneTaq® Hot Start 2X Master Mix with GC Buffer

Order OneTaq® Hot Start 2X Master Mix with Standard Buffer

Order OneTaq® Hot Start DNA Polymerase

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

Order OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

Order OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

Order Quick-Load® Taq 2X Master Mix

Order Standard Taq (Mg-free) Reaction Buffer Pack

Order Standard Taq Reaction Buffer Pack

Order Taq 2X Master Mix

Order Taq 5X Master Mix

Order Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

Order Taq DNA Polymerase with Standard Taq Buffer

Order Taq DNA Polymerase with ThermoPol® Buffer

Order Taq PCR Kit

Order ThermoPol® II (Mg-free) Reaction Buffer Pack

Order ThermoPol® Reaction Buffer Pack

LongAmp® DNA Polymerases

Order LongAmp® Hot Start Taq 2X Master Mix

Order LongAmp® Hot Start Taq DNA Polymerase

Order LongAmp® Taq 2X Master Mix

Order LongAmp® Taq DNA Polymerase

Order LongAmp® Taq PCR Kit

Luna® qPCR & RT-qPCR Products

Order Luna® Universal One-Step RT-qPCR Kit

Order Luna® Universal Probe One-Step RT-qPCR Kit

Order Luna® Universal Probe qPCR Master Mix

Order Luna® Universal qPCR Master Mix

Order LunaScript™ RT SuperMix Kit

Other PCR Polymerases

Order Deep Vent® (exo-) DNA Polymerase

Order Deep Vent® DNA Polymerase

Order Hemo KlenTaq®

Order Vent® (exo-) DNA Polymerase

Order Vent® DNA Polymerase

Phusion® High-Fidelity DNA Polymerases

Order Phusion® GC Buffer Pack

Order Phusion® HF Buffer Pack

Order Phusion® High-Fidelity DNA Polymerase

Order Phusion® High-Fidelity PCR Master Mix with GC Buffer

Order Phusion® High-Fidelity PCR Master Mix with HF Buffer

Order Phusion® Hot Start Flex 2X Master Mix

Order Phusion® Hot Start Flex DNA Polymerase

Order Phusion® High-Fidelity PCR Kit

Master Mixes

Order Hot Start Taq 2X Master Mix

Order LongAmp® Hot Start Taq 2X Master Mix

Order OneTaq^® Quick-Load^® DNA Polymerase

Order OneTaq^® Quick-Load^® DNA Polymerase

Order EpiMark^® Hot Start Taq DNA Polymerase

Order ThermoPol^®Reaction Buffer Pack

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Hot Start Taq DNA Polymerase

Order LongAmp^® Taq 2X Master Mix

Order LongAmp^® Taq DNA Polymerase

Order LongAmp^® Taq PCR Kit

Order LunaScript^™ RT SuperMix Kit

Order Deep Vent^® (exo-) DNA Polymerase

Order Deep Vent^® DNA Polymerase

Order Vent^® (exo-) DNA Polymerase

Order Vent^®DNA Polymerase

Order LongAmp^® Hot Start Taq 2X Master Mix

Order LongAmp^® Taq 2X Master Mix

Order Bst 2.0 WarmStart^® DNA Polymerase

Order WarmStart^® Colorimetric LAMP 2X Master Mix (DNA & RNA)

Order WarmStart^® LAMP Kit (DNA & RNA)