RNase Inhibitor, Murine

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0314S     -20       RNase Inhibitor, Murine M0314SVIAL -20 1 x 0.075 ml 40,000 units/ml
M0314L     -20       RNase Inhibitor, Murine M0314LVIAL -20 1 x 0.375 ml 40,000 units/ml

Application Features

• RT-PCR
• cDNA synthesis
• In vitro transcription/translation
• Enzymatic RNA labeling reaction
• Other applications where the integrity of RNA is important

1. Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNase Inhibitor molecules which have complexed with a ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided.
2. The recommended concentration of RNase Inhibitor in a reaction is 1 unit/μl. During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination (i.e. enzymes, plasmid from a mini prep.)

1. Kim, B.M. et al. Protein Science. 8, 430-434.

1. Protocol for Avoiding Rnase Contamination using Murine Rnase Inhibitor (M0314)
2. Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0314S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0314S_L_v1_0071405
• M0314S_L_v1_0101411
• M0314S_L_v1_0111503
• M0314S_L_v1_0111505
• M0314S_L_v1_0161508
• M0314S_L_v1_0121601
• M0314S_L_v1_0201606
• M0314S_L_v1_0271701
• M0314S_L_v1_0261611
• M0314S_L_v1_0271704
• M0314S_L_v1_0311708
• M0314S_L_v1_0331802
• M0314L_v1_10009659
• M0314L_v1_10018729
• M0314S_v1_10016591
• M0314S_v1_10021645
• M0314L_v1_10027195
• M0314L_v1_10031721
• M0314S_v1_10037465
• M0314L_v1_10037467
• M0314L_v1_10046913
• M0314S_v1_10046912
• M0314L_v1_10052579
• M0314S_v1_10052578
• M0314L_v1_10056314
• M0314L_v1_10061018
• M0314L_v1_10064156
• M0314L_v1_10066832
• M0314L_v1_10067814
• M0314S_v1_10064158
• M0314L_v1_10067911
• M0314L_v1_10071220
• M0314S_v1_10073051
• M0314L_v1_10073044
• M0314L_v1_10079148
• M0314S_v1_10082738
• M0314L_v1_10082920
• M0314S_v1_10085858
• M0314L_v1_10089187
• M0314S_v1_10089188
• M0314L_v1_10094134
• M0314S_v1_10097157
• M0314L_v1_10097156
• M0314L_v1_10106608
• M0314L_v1_10111205
• M0314S_v1_10111206
• M0314L_v1_10115350
• M0314L_v1_10121355
• M0314S_v1_10121437
• M0314L_v1_10126394
• M0314L_v1_10130309
• M0314L_v1_10139355
• M0314S_v1_10137589
• M0314L_v1_10144159
• M0314L_v1_10149076
• M0314L_v1_10150802
• M0314S_v1_10150800
• M0314L_v1_10160882
• M0314S_v1_10153420
• M0314L_v1_10164846
• M0314S_v1_10164844
• M0314L_v1_10176985
• M0314L_v1_10178553
• M0314S_v1_10183992
• M0314L_v1_10183129
• M0314S_v1_10190442
• M0314L_v1_10190443
• M0314L_v1_10204317
• M0314S_v1_10203267
• M0314L_v1_10204828

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• RNase Inhibitor, Murine

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.