WarmStart^® LAMP Kit (DNA & RNA)

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E1700S     -20       WarmStart LAMP 2X Master Mix M1700SVIAL -20 1 x 1.25 ml 2 X   LAMP Fluorescent Dye B1700AVIAL -20 1 x 0.05 ml 50 X
E1700L     -20       WarmStart LAMP 2X Master Mix M1700SVIAL -20 5 x 1.25 ml 2 X   LAMP Fluorescent Dye B1700SVIAL -20 1 x 0.25 ml 50 X

Companion Products

• LAMP Fluorescent Dye
• Bst 2.0 WarmStart DNA Polymerase
• WarmStart® RTx Reverse Transcriptase
• Isothermal Amplification Buffer Pack
• Deoxynucleotide (dNTP) Solution Mix
• Bst 3.0 DNA Polymerase

1. Notomi, T., et al. (2000). Nucleic Acids Res. 28, PubMedID: E63
2. Nagamine, K., et al. (2001). Clin. Chem. 47, 1742-1743.
3. Nagamine, K., Hase, T., Notomi, T. (2002). Mol. Cell Probes. 16, 223-229.
4. Mori, Y., Nagamine, K., Tomita, N., Notomi, T. (2001). Biochem. Biophys. Res. Commun. 289, 150-154.
5. Mori, Y., Kitao, M., Tomita, N., Notomi, T. (2004). J. Biochem. Biophys. Methods. 59, 145-157.
6. Tomita, N., Mori, Y., Kanda, H., Notomi, T. (2008). Nat. Protoc. 3, 877-882.
7. Tanner, N.A. and Evans, T. C. Jr (2014). Curr. Protoc. Mol. Biol. 105, Unit 15.14.
8. Hsieh et al. (2014). Chem. Comm. 50, 3747-3749.
9. Tanner, N.A., Zhang, Y. and Evans, T.C. Jr (2015). Biotechniques. 58, 59-68.

1. WarmStart LAMP Kit (DNA & RNA) Protocol (E1700)

• Optimized Integration of New England Biolabs^® Loop-mediated Isothermal Amplification (LAMP) Reagents with Axxin ISO Instruments

1. What components are included in the WarmStart LAMP Kit (DNA & RNA)?
2. What is the optimal LAMP/RT-LAMP amplification temperature?
3. What is the Mg++ concentration in the WarmStart LAMP/RT-LAMP Master Mix?
4. How do I use the fluorescent dye?
5. What types of input samples/materials are compatible with the LAMP/RT-LAMP mixes?
6. How fast should I expect a result?
7. How do I confirm a positive result?
8. Can I set up my LAMP/RT-LAMP reactions at room temperature?
9. Can I use dUTP and UDG for carryover contamination prevention with the WarmStart LAMP Master Mix?
10. The WarmStart LAMP Master Mix has some precipitation in the tube after thawing, is this normal?
11. How do I design LAMP Primers?
12. What is the difference between NEB #E1708: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) and NEB #E1700: WarmStart LAMP Kit (DNA & RNA)?
13. Amplification occurred in my NTC sample(s) following isothermal incubation. What happened?
14. What detection methods can be used for LAMP/RT-LAMP experiments?
15. What are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
16. Is a separate incubation step required for RT-LAMP?
17. Does NEB have a master mix for LAMP or RT-LAMP reactions?
18. What is LAMP and RT-LAMP?
19. What type of purification is recommended for LAMP primers?

• Using aptamers to control enzyme activities: Hot Start Taq and beyond
• Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E1700S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E1700S_L_v1_0051803
• E1700L_v1_10015905
• E1700S_v1_10015263
• E1700L_v1_10036139
• E1700S_v1_10035550
• E1700S_v1_10037249
• E1700L_v1_10041509
• E1700S_v1_10041510
• E1700L_v2_10045295
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• E1700S_v2_10045662
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• E1700L_v2_10052320
• E1700L_v2_10056358
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• E1700S_v2_10068971
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• E1700L_v2_10073590
• E1700S_v2_10071582
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• E1700L_v2_10074375
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• E1700L_v2_10074440
• E1700L_v2_10074871
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• E1700S_v2_10075340
• E1700S_v2_10076849
• E1700L_v2_10076848
• E1700L_v2_10083971
• E1700S_v2_10082682
• E1700L_v2_10088160
• E1700L_v2_10089589
• E1700L_v2_10091295
• E1700S_v2_10088965
• E1700L_v2_10092679
• E1700L_v2_10094246
• E1700S_v3_10094015
• E1700L_v3_10095915
• E1700L_v3_10099435
• E1700L_v3_10102697
• E1700L_v3_10104085
• E1700S_v3_10103248
• E1700S_v3_10109758
• E1700L_v3_10107128
• E1700S_v3_10110155
• E1700S_v3_10117355
• E1700L_v3_10117586
• E1700S_v3_10127748
• E1700L_v3_10133020
• E1700L_v3_10136877
• E1700S_v3_10145376
• E1700L_v3_10141226
• E1700S_v3_10149829
• E1700S_v3_10137969
• E1700S_v3_10154965
• E1700L_v3_10155252
• E1700S_v3_10160550
• E1700S_v3_10164978
• E1700L_v3_10164979
• E1700S_v3_10170084
• E1700L_v3_10170230
• E1700L_v3_10177217
• E1700S_v3_10175430
• E1700L_v3_10181979
• E1700L_v3_10184940
• E1700S_v3_10206673
• E1700L_v3_10204108
• E1700S_v3_10220979
• E1700L_v3_10221459
• E1700L_v3_10211641
• E1700S_v3_10226845
• E1700L_v3_10226844
• E1700S_v3_10238345
• E1700L_v3_10237752
• E1700S_v3_10245966
• E1700S_v3_10256841
• E1700L_v3_10256840

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• WarmStart LAMP 2X Master Mix
• LAMP Fluorescent Dye

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased product to perform loop-mediated amplification (“LAMP”) and reverse transcription loop-mediated amplification (“RT-LAMP”) for research use only. LAMP is a patented technology belonging to Eiken Chemical Co., Ltd and any other use other than research may require a license from Eiken Chemical Co., Ltd. 

Nucleic acid-based aptamers for use with thermophilic DNA polymerases and reverse transcriptases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the WarmStart^® LAMP Kit (DNA & RNA) for Research Use Only (RUO).  Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.