Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
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Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Bst 3.0 DNA Polymerase is an
in silico designed homologue of Bacillus
stearothermophilus DNA Polymerase I, Large
Fragment engineered and fused to a novel nucleic acid binding domain for improved isothermal
amplification performance and increased reverse
transcription activity. Bst 3.0 DNA Polymerase
contains 5´→3´ DNA polymerase activity with
either DNA or RNA templates and strong strand
displacement activity, but lacks 5´→3´ and 3´→5´
exonuclease activity. Bst 3.0 DNA Polymerase
demonstrates robust performance even in high
concentrations of amplification inhibitors, including dUTP and
features significantly increased reverse transcriptase
activity compared to Bst DNA Polymerase.
An example of the utility and speed offered by Bst 3.0 DNA Polymerase can be seen in this publication that explores the development of a novel digital LAMP assay: Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.
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