Random Primer 9

A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2).

Oligolabeling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).


5' d(N9) 3' [N = A,C,G,T]

Catalog # Concentration Size List Price Your Price Quantity
S1254S Not Applicable 1 A260 units $114.00
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    5' d(N9) 3' [N = A,C,G,T]
    This product is related to the following categories:
    RT-PCR Products,
    Reverse Transcriptases & RT-PCR Products,
    cDNA Synthesis & Reverse Transcriptases Products
    This product can be used in the following applications:
    RT-PCR & cDNA Synthesis,
    RT-qPCR, RT-PCR and cDNA Synthesis,
    RNA Modification,
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