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Bst 2.0 WarmStart DNA Polymerase (Glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows.
Bst 2.0 WarmStart DNA Polymerase is an in silico designed homolog of Bacillus stearothermophilus DNA Polymerase I, Large Fragment (Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. At temperatures above 45°C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. Bst 2.0 WarmStart DNA Polymerase displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.
This product is a core component of the LyoPrime WarmStart® Fluorescent LAMP/RT-LAMP Mix (with UDG), a lyophilized LAMP mix offering ambient/room temperature storage and shipping and streamlined assay development. To learn about the lyophilization process, please visit www.neb.com/lyoprime.
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