Even more from less, for RNA
Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of strand-specific RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries and enabling use of lower input amounts and fewer PCR cycles. The Ultra II Directional RNA kit uses the “dUTP method” (1, 2) for strand-specificity, and has a streamlined, automatable workflow.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext® Ligation Enhancer||-20|
|NEBNext First Strand Synthesis Reaction Buffer||-20|
|NEBNext Second Strand Synthesis Enzyme Mix||-20|
|NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix||-20||10 X|
|NEBNext USER® Enzyme||-20|
|NEBNext Ultra II End Prep Enzyme Mix||-20|
|NEBNext Ultra II End Prep Reaction Buffer||-20|
|NEBNext® Ultra™ II Ligation Master Mix||-20|
|NEBNext® Ultra II Q5® Master Mix||-20||2 X|
|NEBNext First Strand Synthesis Enzyme Mix||-20|
|NEBNext Adaptor Dilution Buffer||-20||1 X|
|(0.1X) TE Buffer||-20||0.1 X|
|NEBNext Strand Specificity Reagent||-20|
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