Ideally, a library will include each transcript completely, from the 5´ to 3´ end. If workflows are less efficient, incomplete coverage of transcripts can occur, especially when lower input amounts or low-quality RNA are used.
Improve uniformity of coverage, at a range of input amounts
Gene body of transcripts
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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.