Ideally, a library will include each transcript completely, from the 5´ to 3´ end. If workflows are less efficient, incomplete coverage of transcripts can occur, especially when lower input amounts or low-quality RNA are used.

Improve uniformity of coverage, at a range of input amounts

Gene body of transcripts

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Poly(A) mRNA enrichment workflow

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rRNA depletion workflow

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rRNA depletion with FFPE workflow

Individual transcripts

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Poly(A) mRNA enrichment workflow

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rRNA depletion with FFPE workflow

 

ERCC spike-ins

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Poly(A) mRNA enrichment workflow

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Poly(A) mRNA enrichment workflow

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rRNA depletion workflow

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems

Available kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

NEBNext_portal_product_icon 

NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.