FAQ: I have USER enzyme from my library prep kit (blue cap) and USER enzyme from my NEBNext Index Primer kit (red cap). Are they the same?

Yes, it is the same. We provide USER in the NEBNext Ultra RNA Directional kits since it is needed for the workflow (blue cap). USER enzyme with the red cap is provided in our oligo kits for use with the NEBNext adaptor. If you are using NEBNext Ultra I or Ultra II Directional RNA Library prep kit with NEBNext adaptor only one USER step is required and either vial of USER enzyme provided can be used.