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Library yield is one important measure of library preparation success, and the revision of each step in the RNA library prep workflow enables substantially higher yields with the NEBNext Ultra II Directional RNA Library Prep Kit compared to earlier NEBNext kits, as well as other commercially available kits. Achieving sufficient yields from low input amounts can be especially challenging. However, the high efficiencies of the NEBNext Ultra II Directional workflow overcomes these challenges, and users can now obtain higher library yields with lower input amounts for RNA, prepared by poly(A) mRNA enrichment or rRNA depletion workflows. Further, samples of low quality, such as FFPE RNA, can also be used successfully, especially in conjunction with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat).

Generate the highest yields, from a wide range of input amounts

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Poly(A) mRNA enrichment workflow
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rRNA depletion workflow
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rRNA depletion with FFPE workflow

NEBNext Ultra II Directional RNA generates yields several fold higher than the original Ultra Directional RNA

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Poly(A) mRNA enrichment workflow
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rRNA depletion workflow
 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems

Available kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.