FFPE RNA can be of very low quality, due to damage occurring during both the fixation process and subsequent storage. Further, it is often available only in very low quantities, creating an even more difficult challenge for library preparation. The NEBNext rRNA Depletion Kit is ideally suited for use with low quality samples such as FFPE RNA, due its complete tiling of rRNA probes, and high-efficiency RNase H-based method. Using this in combination with the NEBNext Ultra II Directional RNA Library Prep Kit, provides a high-performance library preparation workflow for even low quality FFPE RNA samples.

Generate robust libraries for FFPE RNA samples

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Yield

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Strandedness / directionality

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rRNA levels remaining

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Duplication rates

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GC content distribution

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Library complexity

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Transcript coverage

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Individual transcript coverage

 

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

ILLUMINA®, TRUSEQ® and NEXTSEQ® are registered trademarks of Illumina, Inc.
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.
RIBO-ZERO™ is a trademark of Illumina, Inc.
KAPA™ is a trademark of Kapa Biosystems

Available kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.

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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.