NEBNext® Poly(A) mRNA Magnetic Isolation Module

Description











The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext RNA Binding Buffer (2X)42X
NEBNext Wash Buffer4
Nuclease-free Water-20
NEBNext Tris Buffer4
NEBNext Oligo d(T)25 Beads4

Advantages and Features

Applications

Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

Properties and Usage

Materials Required but not Supplied

  • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
  • Magnetic Rack (Alpaqua, cat #A001322 or equivalent)
  • Thermal cycler or heat block
  • Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.

Storage Temperature

4°C

FAQs

  1. Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
  2. Do I need two rounds of binding?
  3. Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?

Protocols

  1. Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Usage Guidelines & Tips

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.