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  • NEBNext® Poly(A) mRNA Magnetic Isolation Module

    Description

    The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    NEBNext Oligo d(T)25 Beads4
    NEBNext RNA Binding Buffer (2X)42X
    NEBNext Wash Buffer4
    Nuclease-free Water-20
    NEBNext Tris Buffer4

    Advantages and Features

    Applications

    Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

    Properties and Usage

    Materials Required but not Supplied

    • 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
    • Magnetic Rack (Alpaqua, cat #A001322 or equivalent)
    • Thermal cycler or heat block

    Storage Temperature

    4°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].
    1. Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
    2. Do I need two rounds of binding?
    3. Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
    1. Isolate mRNA using the NEBNext Oligo d(T)25 Magnetic Beads (E7490)