No. The kit can be used with the NEBNext Multiplex (NEB #E7335, #E7500, #E7710, #E7730, #E7600, #E6609) and Singleplex (NEB #E7350) Oligos for Illumina. For use with the Dual Index Primers (NEB #E7600), consult the NEB #E7600 manual for valid barcode combinations and tips for setting up PCR reactions.
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