Fast Cloning

Fast cloning reagents from NEB:

SEAMLESS CLONING

NEBuilder^® HiFi DNA Assembly Master Mix, NEBuilder HiFi DNA Assembly Cloning Kit & NEBuilder HiFi DNA Assembly Bundle for Large Fragments

Rapid, seamless assembly/cloning of multiple fragments in as little as 15 minutes

NEB^® Gibson Assembly Master Mix &
Gibson Assembly Cloning Kit

Successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility

NEBridge Golden Gate Assembly Kit (BsmBI-v2)

Efficient, ordered assembly of multiple fragments in a single reaction

DIGESTION

High-Fidelity (HF^®) restriction enzymes

HF enzymes offer reduced star activity, rapid digestion and 100% activity in rCutSmart™ Buffer

Time-Saver™ restriction enzymes

>180 enzymes are Time-Saver qualified and will digest DNA in 5-15 minutes; digest overnight with no unwanted star activity

END MODIFICATION

Quick CIP

Complete dephosphorylation in 10 minutes with rapid, irreversible heat inactivation

Quick Blunting Kit

Blunt restriction enzyme-digested DNA in less than 30 minutes at room temperature

AMPLIFICATION

Q5^® High-Fidelity DNA Polymerases

With the lowest error rate of all known commercially available PCR polymerases, Q5 enables extension times as low as 10 seconds per kb on templates up to 20 kb

PURIFICATION

Monarch^® DNA Purification kits

Purify high-quality DNA in minutes (5 minutes for the PCR & DNA Cleanup Kit!). Designed with sustainability in mind, Monarch kits use less plastic and responsibly-sourced packaging

LIGATION

Instant Sticky-end Ligase Master Mix

Specially formulated to instantly ligate 2-4 basepair overhangs! Just add DNA, flick the tube, and ligation occurs instantly! No need for incubation.

Blunt/TA Ligase Master Mix

Specially formulated to ligate single-base and blunt ends in just 15 minutes

Quick Ligation Kit

Ligates both cohesive and blunt ends in just 5 minutes at room temperature

TRANSFORMATION

NEB Turbo Competent E. coli (High-Efficiency)

Generate colonies in as little as 6.5 hours with a 5-minute transformation protocol

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

Featured fast cloning resources:

ONLINE TOOLS

NEBcloner^®

For products, protocols and tips for each step of your traditional cloning experiment

NEBioCalculator^®

For help with scientific calculations and conversions for DNA and RNA

NEBcutter^®

To identify the restriction sites within your DNA sequence

BROCHURES & TECHNICAL GUIDES

Molecular Cloning Technical Guide

Get help with product selection, protocols, tips for optimization and troubleshooting.

Videos

Overview of Traditional Cloning

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly into vectors and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more in this tutorial about the benefits and disadvantages of Traditional Cloning.
NEBUILDER^® HiFi: the next generation of DNA Assembly

In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary.
Golden Gate Assembly Workflow

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.
Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly^® or Gibson Assembly^®

Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.
TIME-SAVER™ Qualified Restriction Enzymes

How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist.
DNA Blunting Tutorial

The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how.
Cloning With Restriction Enzymes

Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment.
Tips for Successful Transformations with NEB^® Competent Cells

Follow these tips to get superior results.
Why Choose Q5^® High-fidelity Polymerase?

Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase".
Monarch^® PCR & DNA Cleanup Kit Protocol

Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).

Fast cloning reagents from NEB:

Featured fast cloning resources:

Videos

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