Quick DNA Ligase will seal nicks for these substrates:
The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C )
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature . (25°C )For details on NEB's quality controls for DNA ligases, visit our Ligase Quality page.
1X Quick Ligation Reaction Buffer:
66 mM Tris-HCl
10 mM MgCl2
1 mM Dithiothreitol
1 mM ATP
7.5% Polyethylene glycol (PEG6000)
pH 7.6 @ 25°C
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Quick Ligation Reaction Buffer||-20||2 X|
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