Quick Ligation™ Kit
The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25°C )
- Fast - 5 minutes for cohesive or blunt ends
- Convenient - ligation performed at room temperature
- Flexible - suitable for all common ligation reactions
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Quick Tips - Do I need a 5' phosphate for ligation?
Quick Tips - Electroporation after ligation
Quick Tips - Troubleshooting problematic ligation reactions
Quick Tip - What is the ideal incubation temperature for ligation?
Quick Tip - When is a long incubation time necessary for ligation?
What are the best conditions for DNA ligation?
What molar ratios should I use for DNA Ligation?
How do I choose the best DNA Ligase?
Ordering InformationDid you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options.
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature . (25°C )For details on NEB's quality controls for DNA ligases, visit our Ligase Quality page.
1X Quick Ligation Reaction Buffer:.
66 mM Tris-HCl
10 mM MgCl2
1 mM Dithiothreitol
1 mM ATP
7.5% Polyethylene glycol (PEG6000)
pH 7.6 @ 25°C
The following reagents are supplied with this product:
Store at (°C) Concentration Quick Ligation Reaction Buffer -20 2 X Quick Ligation™ Kit -20
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