Monarch^® Total RNA Miniprep Kit

The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol^® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A_260/280 and A_260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.

Workflow for the Monarch Total RNA Miniprep Kit

Properties:

Tested Sample Types:

Additional Data Figures:

Figure 1. Monarch-purified RNA is high-quality and compatible with a wide variety of downstream applications

Figure 2. The Monarch Total RNA Miniprep Kit can generate high quality RNA from as few as 100 HeLa cells

Figure 3. The Monarch Total RNA Miniprep Kit successfully purifies small RNAs below 200 nucleotides, enabling a more faithful representation of the total RNA pool

This product is related to the following categories:

Total RNA Extraction & Purification Products,

RNA Extraction and Purification,

Nucleic Acid Purification Products