
Total RNA Extraction & Purification
RNA plays a key role in gene expression and regulation. Isolation, extraction and purification of RNA from biological samples is an essential first step in the study of many biological processes. RNA extraction starts with homogenization of the samples followed by lysis of the cells to release the RNA. RNA is then conditioned to bind to a capture column and washed to remove salts and other contaminants before it is eluted. Any contaminating genomic DNA can be removed with column-based or enzyme-based methods.
The Monarch Spin RNA Isolation Kit is a universal kit for total RNA extraction and purification from a wide variety of sample types including blood, cells, tissues, and tough-to-lyse samples (including bacterial and yeast). The kit includes a protection reagent for sample storage and lysis, Proteinase K for homogenization of some sample types, as well as gDNA removal columns and DNase I for gDNA removal.
The Monarch Spin RNA Isolation Kit purifies RNA ranging in size from full length RNA to intact miRNAs. This kit can be used to clean up RNA after enzymatic reactions before use in downstream workflows, including cDNA synthesis, RT-PCR, RT-qPCR and RNA-seq.
Purify high-quality RNA from multiple sample types and low input amounts using the Monarch Spin RNA Isolation Kit

Purify total RNA of all sizes, including RNA <200 nts with the Monarch Spin RNA Isolation Kit

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- Is the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110) compatible with Luna RT-qPCR Reagents?
- Is the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110) compatible with NEBNext reagents for RNA Library Prep?
- What is the lowest volume in which I can elute my RNA with the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)?
- When using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110), how can I preserve my sample if I am not ready to extract RNA right after harvest?
- I see genomic DNA in my preps when using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110). What could be the problem?
- Can I use the Monarch Spin RNA Isolation Kit (Mini) for RNA (NEB #T2110) cleanup after enzymatic manipulations?
- Can I skip the on-column DNase I treatment step of the Monarch Spin RNA Isolation Kit (Mini) protocol (NEB #T2110)?
- Can the genomic DNA captured on the Monarch Spin Columns S2C (NEB #T3017) be eluted and used in downstream applications?
- Protocol for Total RNA Isolation and Purification Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Protocol for Sample Lysis of Bacteria or Yeast Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Protocol for Sample Lysis of Cells Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Protocol for Sample Lysis of Plants or Insects Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Protocol for Sample Lysis of Tissues or Leukocytes Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Protocol for RNA Reaction Cleanup Using the Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Monarch RNA Purification Brochure
- Troubleshooting Guide for Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)
- Assessing RNA (NEB #T2110)
- Choosing Sample Input Types for RNA Purification and Average Yields Expected
Brochures
Troubleshooting Guides
Usage Guidelines
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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