Monarch^® RNA Lysis Buffer

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T2012L     25       Monarch® RNA Lysis Buffer T2012-1 25 2 x 50 ml Not Applicable

Companion Products

• Luna^® Universal One-Step RT-qPCR Kit
• Luna^® Universal Probe One-Step RT-qPCR Kit
• ProtoScript® II First Strand cDNA Synthesis Kit
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext® Poly(A) mRNA Magnetic Isolation Module
• ProtoScript® II Reverse Transcriptase
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Monarch® RNA Purification Columns
• Monarch^® DNA/RNA Protection Reagent
• Monarch^® gDNA Removal Columns
• Monarch^® Collection Tubes II
• Monarch^® Total RNA Miniprep Kit
• Monarch^® RNA Priming Buffer
• Monarch^® RNA Wash Buffer
• Monarch^® Total RNA Miniprep Enzyme Pack
• Nuclease-free Water

1. Download Quick Protocol Card T2010
2. Quick Protocol for Total RNA Extraction (NEB #T2010)
3. Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit

• General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
• Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
• Guidelines for Purification of RNA from Cultured Mammalian Cells
• Guidelines for RNA Purification from Bacteria
• Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
• Guidelines for RNA Purification from Plants
• Guidelines for RNA Purification from Tissues 
• Guidelines for RNA Purification from Whole Blood
• Guidelines for RNA Purification from Yeast

• Purification of synthetic SARS-CoV-2 viral RNA from biological samples using the Monarch^® Total RNA Miniprep Kit and the Monarch RNA Cleanup Kit

1. Can I lyse my adherent cells directly in their wells when using the Monarch Total RNA Miniprep Kit?
2. Can extraction of RNA with the Monarch kits be automated?
3. Is live SARS-CoV-2 virus inactivated during extraction with the Monarch Total RNA Miniprep Kit?

• Troubleshooting Guide for Total RNA Extraction & Purification

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2012L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2012L_v1_10044097
• T2012L_v1_10053943
• T2012L_v1_10074653
• T2012L_v1_10079499
• T2012L_v2_10097584
• T2012L_v2_10141107
• T2012L_v2_10144570
• T2012L_v2_10166738

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® RNA Lysis Buffer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.