For maximal recovery, we recommend eluting RNA with 50-100 µl of nuclease-free water in order to completely wet the membrane. However, elution in 30 µl provides >80% recovery. You can also elute with a lower volume for a more concentrated RNA sample, but recovery will decrease. If using less than 50 µl for elution, it is important to pipet the nuclease-free water directly to the center of the membrane during the elution.
