This product was discontinued on February 6, 2026. A new version of this product is now available. Migrate to the new Monarch StabiLyse™ DNA/RNA Buffer. (NEB #T2111)
Monarch® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. It is a solution for the preservation of nucleic acids in various tissue types. It is supplied as a 2X concentrate. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit.
Figure 1: Monarch DNA/RNA Protection Reagent preserves RNA integrity for up to 1 month at 4°C
Samples were incubated in Monarch DNA/RNA Protection Reagent (NEB #T2011) at 4°C for the indicated time and subsequently processed using the Monarch Total RNA Miniprep Kit (NEB #T2010). Samples were eluted in 100 µl nuclease-free water. Rat liver and blood samples were diluted 5-fold (HeLa cells were not diluted), and 1 µl of each was loaded onto a Bioanalyzer Nano chip (HeLa and rat liver) or Pico chip (blood). The RIN values demonstrate the ability of the Protection Reagent to preserve RNA integrity over time.
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Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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