Monarch^® gDNA Removal Columns

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
T2017L     25       Monarch® gDNA Removal Columns T2017-1 25 2 x 50 columns Not Applicable   Monarch® Spin Collection Tubes T2118-1 25 2 x 50 tubes Not Applicable

Companion Products

• Luna^® Universal One-Step RT-qPCR Kit
• Luna^® Universal Probe One-Step RT-qPCR Kit
• ProtoScript® II First Strand cDNA Synthesis Kit
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
• NEBNext® Poly(A) mRNA Magnetic Isolation Module
• ProtoScript® II Reverse Transcriptase
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Monarch® RNA Purification Columns
• Monarch^® DNA/RNA Protection Reagent
• Monarch^® Total RNA Miniprep Kit
• Monarch^® Collection Tubes II
• Monarch^® RNA Lysis Buffer
• Monarch^® RNA Priming Buffer
• Monarch^® RNA Wash Buffer
• Monarch^® Total RNA Miniprep Enzyme Pack
• Nuclease-free Water

1. Quick Protocol for Total RNA Extraction (NEB #T2010)
2. FFPE Tissue Deparaffinization and Subsequent RNA Purification Using the Monarch Total RNA Miniprep Kit  (NEB #T2010)
3. Total RNA Purification from Cultured Mammalian Cells using the Monarch Total RNA Miniprep Kit (NEB #T2010)
4. Total RNA Purification from Tissues and Leukocytes using the Monarch Total RNA Miniprep Kit (NEB #T2010)
5. Total RNA Purification from Tough-to-Lyse Samples (bacteria, yeast, plant, etc.) using the Monarch Total RNA Miniprep Kit (NEB #T2010)

• General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
• Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
• Guidelines for Purification of RNA from Cultured Mammalian Cells
• Guidelines for RNA Purification from Bacteria
• Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
• Guidelines for RNA Purification from Plants
• Guidelines for RNA Purification from Tissues 
• Guidelines for RNA Purification from Whole Blood
• Guidelines for RNA Purification from Yeast
• Guidelines for Sample Stabilization Using the Monarch DNA/RNA Protection Reagent

1. Can I skip the gDNA removal column step of the Monarch Total RNA Miniprep Kit protocol?
2. Can the genomic DNA captured on the Monarch gDNA Removal Column (NEB #T2017) be eluted and used in downstream applications?
3. Is the Monarch Total RNA Miniprep Kit compatible with Luna RT-qPCR Reagents?
4. Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep?
5. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from bacteria?
6. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from plants?
7. Can the Monarch Total RNA Miniprep Kit be used to purify RNA from yeast?
8. What is the lowest volume in which I can elute my RNA with the Monarch Total RNA Miniprep Kit (NEB #T2010)?
9. I noticed that in the Monarch Total RNA Miniprep Kit (NEB #T2010), the Monarch gDNA Removal Columns (NEB #T2017) are now tinted orange. Does this impact performance?
10. I notice that I'm applying force to fit the column into a collection tube. Should I be doing this? What collection tubes are compatible with columns?
11. Are the Monarch gDNA Removal Columns (NEB #T2017) and Monarch RNA Purification Columns (NEB #T2007) included with the Monarch Total RNA Miniprep Kit (NEB #T2010) compatible with the QIACube^® System?

• Troubleshooting Guide for Total RNA Extraction & Purification

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• T2017L_v1
• T2017L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2017L_v1_10043831
• T2017L_v1_10044096
• T2017L_v1_10053945
• T2017L_v1_10074654
• T2017L_v1_10079512
• T2017L_v2_10141095
• T2017L_v2_10144562
• T2017L_v2_10166741
• T2017L_v2_10175999
• T2017L_v2_10213958
• T2017L_v2_10247134

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.