As with all Monarch nucleic acid purification kits, the columns and components of the T2010 kit are available for sale separately. The contents of the kit are outlined below, but please note that some sample types do not require all components. A summary of the products needed for processing common sample types is provided below, and the product manual provides detailed information on processing various sample types.
Products Required for Processing Various Sample Types
| Product Name | NEB # | Cultured Mammalian Cells ** | Blood | Tissue or Leukocytes | Saliva+ | Swabs in transport media | Swabs without transport media | Tough-to-Lyse samples |
| Monarch® DNA/RNA Protection Reagent* | T2011 | ✓ (2X) | ✓ (1X) | ✓ (2X) | ✓ (1X) | ✓*** | ||
| Monarch® RNA Lysis Buffer | T2012 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |
| Monarch gDNA Removal Columns | T2017 | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |
| Proteinase K + Associated Buffers | Components of T2019 – Monarch Total RNA Enzyme Pack | ✓ |
✓ |
✓ |
✓ |
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| DNase I + Associated Buffer | Components of T2019 – Monarch Total RNA Enzyme Pack | Optional but recommended for all sample types |
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| Monarch® RNA Purification Columns | T2007 | Required for all sample types | ||||||
| Monarch® RNA Priming Buffer | T2013 | Required for all sample types | ||||||
| Monarch® RNA Wash Buffer | T2014 | Required for all sample types | ||||||
| Nuclease-free Water | B1500 | Required for all sample types | ||||||
* Protection Reagent is supplied as a 2X concentrate. For liquid samples (e.g. blood) use the 2X concentrate, for other samples, prepare a 1X dilution.
** Protection Reagent is not required for processing cultured mammalian cells. However, cells can be stabilized and stored in Protection Reagent, if desired, and then processed.
*** Protection Reagent is required for mechanical lysis of tough-to-lyse samples. For enzymatic lysis of bacteria and yeast, Protection Reagent is not required
+ For users interested in using the Proteinase K Protocol for saliva samples, Monarch DNA/RNA Protection Reagent and Proteinase K are also required.
