Home Restriction Endonucleases EcoRI

EcoRI

EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. Learn more.

NEB restriction endonuclease that recognizes the sequence G^AATT_C

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF^®) version available (NEB #R3101) supplied with rCutSmart^™ Buffer
Now comes supplied with 1 vial of Gel Loading Dye, Purple (6X)
Restriction Enzyme Cut Site: G/AATTC

Catalog #	Cat #	Concentration	Conc	Size	List Price
R0101S	20,000 units/ml	10,000 units	$73.00	$73.00	Sign In Sign In
R0101T	100,000 units/ml	10,000 units	$73.00	$73.00	Sign In Sign In
R0101L	20,000 units/ml	50,000 units	$301.00	$301.00	Sign In Sign In
R0101M	100,000 units/ml	50,000 units	$301.00	$301.00	Sign In Sign In

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Product Information

EcoRI has a High Fidelity version EcoRI-HF^® (NEB #R3101).

High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.

For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.

Product Source

An E. coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).

This product is related to the following categories:: Restriction Endonucleases C G,; Time-Saver Qualified Restriction Enzymes

This product can be used in the following applications:: Fast Cloning: Accelerate your cloning workflows with reagents from NEB,; Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0101S

-20

EcoRI	R0101SVIAL	-20	1 x 0.5 ml	20,000 units/ml
NEBuffer™ EcoRI/SspI	B0101SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0101T

-20

EcoRI	R0101TVIAL	-20	1 x 0.1 ml	100,000 units/ml
NEBuffer™ EcoRI/SspI	B0101SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0101L

-20

EcoRI	R0101LVIAL	-20	2 x 1.25 ml	20,000 units/ml
NEBuffer™ EcoRI/SspI	B0101SVIAL	-20	2 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0101M

-20

EcoRI	R0101MVIAL	-20	1 x 0.5 ml	100,000 units/ml
NEBuffer™ EcoRI/SspI	B0101SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ EcoRI/SspI
Incubate at 37°C

1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl₂
0.025% Triton® X-100
(pH 7.5 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 50%

Diluent Compatibility
- Diluent C
Storage Buffer

10 mM KPO₄
300 mM NaCl
0.1 mM EDTA
200 µg/ml Recombinant Albumin
50% Glycerol
1 mM DTT
0.15% Triton® X-100
pH 7 @ 25°C

Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping

Isoschizomers

EcoRI
EcoRI-HF
Related Products
Companion Products
- Nuclease-free Water
Materials Sold Separately
- Gel Loading Dye, Purple (6X)
- NEBuffer™ Set (EcoRI/SspI, DpnII)
Product Notes
1. May exhibit star activity in NEBuffer r2.1 or rCutSmart Buffer.
2. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol..
3. Blocked by some combinations of overlapping CpG methylation.

Protocols, Manuals & Usage
- Protocols
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Digest Protocol
  
  Double Digest Protocol with Standard Restriction Enzymes
- Usage Guidelines
  Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
  
  Activity of Restriction Enzymes in PCR Buffers
  
  Cleavage Close to the End of DNA Fragments
  
  Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
  
  Double Digests
  
  Effects of CpG Methylation on Restriction Enzyme Cleavage
  
  Heat Inactivation
  
  NEBuffer Activity/Performance Chart with Restriction Enzymes
  
  Optimizing Restriction Endonuclease Reactions
  
  Restriction Endonucleases - Survival in a Reaction
  
  Restriction Enzyme Diluent Buffer Compatibility
  
  Restriction Enzyme Tips
  
  Single Letter Codes
  
  Site Preferences
  
  Star Activity
  
  Traditional Cloning Quick Guide
Tools & Resources
- Selection Charts
  Alphabetized List of Recognition Sequences
  
  Cleavage of Supercoiled DNA
  
  Compatible Cohesive Ends and Generation of New Restriction Sites
  
  Dam-Dcm and CpG Methylation
  
  Frequencies of Restriction Sites
  
  Isoelectric Points (pI) for Restriction Enzymes
  
  Isoschizomers
  
  NEB Diluent and Buffer Table
  
  Recleavable Filled-in 5' Overhangs
  
  Why Choose Recombinant Enzymes?
- Web Tools
  Competitor Cross-Reference Tool
  
  DNA Sequences and Maps Tool
  
  Double Digest Finder
  
  Enzyme Finder
  
  NEBcutter™ v3.0
  
  REBASE®
FAQs & Troubleshooting
- FAQs
  What is Star Activity and how can it be avoided?
  
  Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
  
  Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  
  Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
- Troubleshooting
  Restriction Enzyme Troubleshooting Guide
Citations & Technical Literature
- Citations
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
  
  Additional Citations
  
  Tong C, Li H, Wang Y, Li X, Ou J, Wang D, Xu H, Ma C, Lang X, Liu G, Zhang B, Shi J. (2016) Construction of High-Density Linkage Maps of Populus deltoides × P. simonii Using Restriction-Site Associated DNA Sequencing. PLoS One; Mar 10;11(3), e0150692.
  
  Yang H, Wei CL, Liu HW, Wu JL, Li ZG, Zhang L, Jian JB, Li YY, Tai YL, Zhang J, Zhang ZZ, Jiang CJ, Xia T, Wan XC. (2016) Genetic Divergence between Camellia sinensis and Its Wild Relatives Revealed via Genome-Wide SNPs from RAD Sequencing. PLoS One; Mar 10;11(3), e0151424.
  
  Mousavi M, Tong C, Liu F, Tao S, Wu J, Li H, Shi J. (2016) De novo SNP discovery and genetic linkage mapping in poplar using restriction site associated DNA and whole-genome sequencing technologies. BMC Genomics; Aug 18;17, 656.
  
  Xiao B, Tan Y, Long N, Chen X, Tong Z, Dong Y, Li Y. (2015) SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing. J Biol Res (Thessalon); Oct 6;22:11. ,
  
  Zhang Q, Liu C, Liu Y, VanBuren R, Yao X, Zhong C, Huang H. (2015) High-density interspecific genetic maps of kiwifruit and the identification of sex-specific markers. DNA Res; Oct;22(5), 367-75.
  
  Bonatelli IA, Carstens BC, Moraes EM. (2015) Using Next Generation RAD Sequencing to Isolate Multispecies Microsatellites for Pilosocereus (Cactaceae). PLoS One; Nov 11;10(11), e0142602.
  
  Fischer G, Azorsa F, Garcia FH, Mikheyev AS, Economo EP. (2015) Two new phragmotic ant species from Africa: morphology and next-generation sequencing solve a caste association problem in the genus Carebara Westwood. Zookeys; Oct 5;(525), 77-105.
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).
  
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  R0101S_L_v3
  
  R0101T_M_v4
  
  R0101S_L_E_v4
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  R0101S_L_v1_0341311
  
  R0101S_L_v1_0331301
  
  R0101S_L_v1_0331305
  
  R0101S_L_v1_0331311
  
  R0101T_M_v1_0331301
  
  R0101T_M_v1_0331305
  
  R0101T_M_v1_0341311
  
  R0101T_M_v1_0341405
  
  R0101S_L_v1_0341409
  
  R0101T_M_v1_0341410
  
  R0101S_L_v1_0341405
  
  R0101S_L_v2_0341412
  
  R0101S_L_v1_0341503
  
  R0101S_L_v2_0341504
  
  R0101T_M_v2_0341504
  
  R0101T_M_v2_0341509
  
  R0101S_L_v2_0341510
  
  R0101S_L_v2_0341602
  
  R0101S_L_v2_0341604
  
  R0101T_M_v2_0341604
  
  R0101T_M_v2_0341610
  
  R0101S_L_v2_0341701
  
  R0101S_L_v2_0341704
  
  R0101T_M_v2_0351706
  
  R0101T_M_v2_0351710
  
  R0101S_L_v2_0351711
  
  R0101T_M_v2_0351801
  
  R0101S_L_v2_0351804
  
  R0101S_v2_10014948
  
  R0101M_v2_10008581
  
  R0101M_v2_10019709
  
  R0101S_v2_10012435
  
  R0101L_v2_10026006
  
  R0101S_v2_10026003
  
  R0101L_v2_10030777
  
  R0101M_v2_10031413
  
  R0101S_v2_10033228
  
  R0101T_v2_10031415
  
  R0101S_v2_10033233
  
  R0101S_v2_10046965
  
  R0101M_v2_10048681
  
  R0101L_v2_10052727
  
  R0101T_v2_10050619
  
  R0101S_v2_10052840
  
  R0101S_v2_10057096
  
  R0101L_v2_10060022
  
  R0101S_v2_10061061
  
  R0101T_v2_10061321
  
  R0101M_v2_10064285
  
  R0101S_v2_10066822
  
  R0101L_v2_10078628
  
  R0101S_v2_10081658
  
  R0101M_v2_10079035
  
  R0101T_v2_10087360
  
  R0101S_v2_10087359
  
  R0101S_v2_10092924
  
  R0101L_v2_10095559
  
  R0101S_v2_10095558
  
  R0101T_v2_10104704
  
  R0101M_v2_10109723
  
  R0101L_v2_10119696
  
  R0101L_v3_10126695
  
  R0101S_v3_10129803
  
  R0101T_v3_10130773
  
  R0101M_v3_10131858
  
  R0101M_v3_10135001
  
  R0101T_v3_10135003
  
  R0101S_v3_10140539
  
  R0101L_v3_10140538
  
  R0101M_v3_10150306
  
  R0101T_v3_10154599
  
  R0101L_v3_10156282
  
  R0101S_v3_10160128
  
  R0101L_v3_10173402
  
  R0101S_v3_10173400
  
  R0101T_v3_10173186
  
  R0101S_v3_10181362
  
  R0101S_v3_10186608
  
  R0101M_v3_10191644
  
  R0101S_v3_10195706
  
  R0101L_v3_10206909
  
  R0101S_v3_10206908
  
  R0101T_v3_10191647
  
  R0101T_v3_10224155
  
  R0101S_v3_10215912
  
  R0101M_v3_10225991
  
  R0101L_v4_10227225
  
  R0101M_v4_10227222
  
  R0101S_v4_10227230
  
  R0101T_v4_10227231
  
  R0101S_v4_10242547
  
  R0101M_v4_10262102
  
  R0101T_v4_10265611
  
  R0101L_v4_10270206
  
  R0101S_v4_10270205
  
  R0101S_v4_10272276
  
  R0101S_v4_10288335
  
  R0101S_v4_10303800
  
  R0101M_v4_10305684
  
  R0101L_v4_10306341
  
  R0101T_v4_10310879
  
  R0101L_v4_10317307
  
  R0101S_v4_10315767
  
  R0101S_v4_10335241
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  EcoRI
  
  NEBuffer™ EcoRI/SspI
  
  Gel Loading Dye, Purple (6X)
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.