NEBuffer™ Set (EcoRI/SspI, DpnII)

4 vials of NEBuffers for restriction enzymes requiring unique buffers ((2 x EcoRI/SspI, 2 x DpnII)

New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.

Set contains two 1.25ml vials of each unique NEBuffer: EcoRI/SspI and DpnII.

1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl₂
0.025% Triton^® X-100
(pH 7.5 @ 25°C)

1X NEBuffer™ DpnII
50 mM Bis-Tris-HCl
100 mM NaCl
10 mM MgCl₂
0.1 mM DTT
(pH 6 @ 25°C)

This product is related to the following categories:

Restriction Endonuclease Buffers & Diluents Products,

Buffers Products

This product can be used in the following applications:

Cloning Ligation