Home Restriction Endonucleases BsmBI-v2

BsmBI-v2

BsmBI-v2 has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10284618. Learn more.

This product replaces BsmBI (NEB #R0580).

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).

NEB Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN and is optimized for Golden Gate Assembly

Isoschizomers | Single Letter Code | Pronunciation:

Time-Saver™ qualified for digestion in 5-15 minutes
Used in Golden Gate Assembly
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Supplied with 1 vial of Gel Loading Dye, Purple (6X) (NEB #B7024)
NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
Can be used with NEBridge^® Ligase Master Mix for Golden Gate Assembly
Restriction Enzyme Cut Site: CGTCTC

Catalog #	Cat #	Concentration	Conc	Size	List Price
R0739S	10,000 units/ml	200 units	$87.00	$87.00	Sign In Sign In
R0739L	10,000 units/ml	1,000 units	$361.00	$361.00	Sign In Sign In

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Product Information

Product Source

An E. coli strain that carries the modified cloned BsmBI gene from Bacillus stearothermophilus B61. BsmBI-v2 is an engineered and improved version of BsmBI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)

This product is related to the following categories:: Restriction Endonucleases B,; Time-Saver Qualified Restriction Enzymes

This product can be used in the following applications:: DNA Preparation,; High-throughput cloning and automation solutions,; DNA Analysis,; NEBridge® Golden Gate Assembly ,; Restriction Enzyme Digestion,

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0739S

-20

BsmBI-v2	R0739SVIAL	-20	1 x 0.02 ml	10,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0739L

-20

BsmBI-v2	R0739LVIAL	-20	1 x 0.1 ml	10,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 55°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 55°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Usage Concentration
1X

Activity in NEBuffers
NEBuffer™ r1.1: <10%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 25%

Diluent Compatibility
- Diluent B
Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
80°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Isoschizomers

BsmBI-v2
Esp3I

Activity at Temperature
@37°C: 10%
Related Products
Companion Products
Materials Sold Separately
- Gel Loading Dye, Purple (6X), no SDS
Product Notes
1. BsmBI-v2 is an isoschizomer of Esp3I