neb31 cloned at NEB recombinant engineered dil_A 55 80 Heat

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at

NEB restriction endonuclease that recognizes the sequence CAGTG_NN^


  • Sourced from an E. coli strain that carries the cloned and modified BtsI gene from Bacillus thermoglucosidasius
  • 100% activity in rCutSmart™ Buffer allowing for easier double digests
  • Not sensitive to dam, dcm or mammalian CpG methylation
  • Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Restriction Enzyme Cut Site: CAGTG(2/0)


Catalog # Concentration Size List Price Your Price Quantity
R0664S 1,000 units/ml 100 units $120.00
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