BclI-HF
High-Fidelity (HF®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:
- Engineered for improved performance
- 100% activity in CutSmart Buffer
- Time-Saver™ qualified for digestion in 5-15 minutes
- Reduced star activity
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Featured Video
Reduce Star Activity with High-Fidelity Restriction Enzymes
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Product Information
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the cloned and modified BclI gene from Bacillus caldolyticus (A. Atkinson).Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration Gel Loading Dye, Purple (6X) 25 6X CutSmart® Buffer -20 10X -
Protocols, Manuals & Usage
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Tools & Resources
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FAQs & Troubleshooting
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Quality, Safety & Legal
Featured Videos
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Reduce Star Activity with High-Fidelity Restriction Enzymes
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Time-Saver Protocol for Restriction Enzyme Digests
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NEB TV Ep. 15 – Applications of Restriction Enzymes
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CutSmart Restriction Enzyme Buffer
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Restriction Enzyme Digest Protocol: Cutting Close to DNA End
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Restriction Enzyme Digestion Problem: DNA Smear on Agarose Gel
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Why is My Restriction Enzyme Not Cutting DNA?
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Restriction Enzyme Digest Problem: Too Many DNA Bands
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Double Digestion with NEBcloner
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