dam^-/dcm^- Competent E. coli

Features

• Dam/Dcm methyltransferase free plasmid growth
  

Application Features

Effect of heat shock time on dam^-/dcm^- competent E. coli transformation efficiency:

Effect of DNA incubation time on dam-/dcm- competent E. coli transformation efficiency: 

Effect of outgrowth medium on transformation efficiency: 

Companion Products

• NEB® Turbo Competent E. coli (High Efficiency)
• NEB® 5-alpha Competent E. coli (High Efficiency)
• NEB® 5-alpha Competent E. coli (Subcloning Efficiency)
• NEB® 5-alpha F' I^q Competent E. coli (High Efficiency)
• NEB® 10-beta Competent E. coli (High Efficiency)
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• SOC Outgrowth Medium

1. CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
2. STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

1. 5 Minute Transformation Protocol (C2925)
2. Transformation Protocol (C2925)

• Additional E. coli Strain Genotypes
• Chemical Transformation Tips
• Genetic Markers
• Making Unmethylated (Dam- Dcm-) DNA
• McrA, McrBC and EcoKI Strain Phenotypes

• Enhancing Transformation Efficiency C2925

• Characteristics of Select E.coli Strains
• Cloning Competent Cell Transformation Selection Chart
• Competent Cell Product Comparison
• Competent Cell Selection Guide
• Strain Properties

• Competitor Cross-Reference Tool

• NEBcloner

1. Can I transform unmethylated DNA into dam-/dcm- competent E.coli (C2925)?
2. Does New England Biolabs offer a methyltransferase free strain of Competent E.coli?
3. How long should I incubate cells on ice after DNA has been added (NEB #C2925H and NEB #C2925I)?
4. Is dam-/dcm- (NEB #C2925H/C2925I) resistant to kanamycin?
5. Is the DNA yield lower using dam-/dcm- strain (C2925)?
6. What are the solutions/recipes (C2925)?
7. What are the strain properties (C2925)?
8. What is the difference between NEB #C2925H and NEB #C2925I?
9. What is the optimal heat shock time for this strain (NEB #C2925H and NEB #C2925I)?
10. Which strain of Competent E. coli should I use for general cloning?
11. Why were the colonies at different size after transformation of dam-/dcm- competent E.coli (C2925)?
12. How should I calculate the transformation efficiency (C2925)?
13. Can I store competent cells at -20°C instead of -80°C?
14. Which kind of transformation tubes should be used?
15. What volume of DNA can be added into competent cells?
16. What is the shelf life for this strain (NEB #C2925H and NEB #C2925I)?
17. Are NEB's competent cells compatible with the “Mix & Go" protocol?
18. How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• C2925H_I_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• C2925H_I_v1_0561607
• C2925H_I_v1_0501502
• C2925H_I_v1_0561608
• C2925H_I_v1_0571608
• C2925H_I_v1_0521508
• C2925H_I_v1_0571610
• C2925H_I_v1_0551609
• C2925H_I_v1_0561610
• C2925H_I_v1_0541611
• C2925H_I_v1_0551611
• C2925H_I_v1_0581611
• C2925H_I_v1_0591611
• C2925H_I_v1_0541701
• C2925H_I_v1_0551701
• C2925H_I_v1_0541703
• C2925H_I_v1_0591703
• C2925H_I_v1_0591704
• C2925H_I_v1_0541705
• C2925H_I_v1_0581706
• C2925H_I_v1_0541603
• C2925H_I_v1_0561604
• C2925H_I_v1_0581707
• C2925H_I_v1_0581708
• C2925H_I_v1_0581710
• C2925H_I_v1_0591707
• C2925H_I_v1_0591708
• C2925H_I_v1_0611706
• C2925H_I_v1_0611709
• C2925H_I_v1_0611710
• C2925H_I_v1_0611712
• C2925H_I_v1_0581712
• C2925H_I_v1_0611801
• C2925H_I_v1_0601801
• C2925H_I_v1_0611803
• C2925H_I_v1_0601803
• C2925H_I_v1_0621803
• C2925I_v1_10011528
• C2925H_v1_10012789
• C2925I_v1_10013424
• C2925I_v1_10015495
• C2925H_v1_10015846
• C2925I_v1_10018135
• C2925H_v1_10020246
• C2925I_v1_10021088
• C2925I_v1_10024318
• C2925H_v1_10024963
• C2925H_v1_10026473
• C2925I_v1_10027603
• C2925H_v1_10029671
• C2925I_v1_10030908
• C2925H_v1_10033407
• C2925I_v1_10033920
• C2925H_v1_10037025
• C2925I_v1_10036364
• C2925H_v1_10041319
• C2925I_v1_10039962
• C2925H_v1_10042684
• C2925I_v1_10043190
• C2925H_v1_10045315
• C2925H_v1_10047054
• C2925I_v1_10046412
• C2925H_v1_10049344
• C2925I_v1_10049970
• C2925H_v1_10052234
• C2925I_v1_10052474
• C2925I_v1_10055256
• C2925H_v1_10056411
• C2925H_v1_10059033
• C2925I_v1_10058462
• C2925I_v1_10061589
• C2925H_v1_10063266

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• dam^-/dcm^- Competent E. coli
• SOC Outgrowth Medium
• pUC19 Vector

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.