dam-/dcm- Competent E. coli
Methyltransferase deficient E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
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Product Information
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation. Highlights
- Grow plasmids free of Dam and Dcm methylation
- Free of animal products
- Phage T1 resistant (fhuA31)
- Transformation efficiency: 1-3 x 106 cfu/μg pUC19 DNA
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- K12 Strain
Genotype
ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1 rspL136 (StrR) dam13::Tn9 (CamR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration SOC Outgrowth Medium 4 1 X pUC19 Vector -20 0.05 ng/μl - Product Categories:
- Cloning Competent Cell Strains Products
- Applications:
- Transformation
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