Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (Hind III digest) in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ 2.1
Incubate at 37°C

1X NEBuffer™ 2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
100 μg/ml BSA
(pH 7.9 @ 25°C)

Activity in NEBuffers

CutSmart® Buffer: 100%
NEBuffer 1.1: 75%
NEBuffer 2.1: 100%
NEBuffer 3.1: 75%

Diluent Compatibility

• Diluent B

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

65°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• NEBuffer 2.1

1. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
2. May exhibit star activity in CutSmart Buffer.
3. BmrI produces DNA fragments that have a single base 3´ extension which are more difficult to ligate than blunt-ended fragments.
4. BmrI cleaves DNA specifically both in the presence and absence of Mg²⁺ ions in the reaction mixture.
5. Active in the presence of EDTA.

1. Do BmrI generated ends ligate well?
2. Is extended digestion of BmrI recommended?
3. My enzyme is no longer Time-Saver™ qualified. What happened?
4. What effect does BSA have on the performance of NEB’s restriction enzymes when included in the new buffers?
5. How can I access the old NEBuffer Activity Chart?
6. Why is my Restriction Enzyme not cutting DNA?
7. Why do I see a DNA smear on an agarose gel after a restriction digest?
8. Why do I see additional DNA bands on my gel after a restriction digest?
9. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?

1. Optimizing Restriction Endonuclease Reactions
2. Double Digest Protocol with Standard Restriction Enzymes

• Alphabetized List of Recognition Specificities
• Buffer and Diluent Formulation Table
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Cross Index of Recognition Sequences
• Dam-Dcm and CpG Methylation
• Enzymes with Nonpalindromic Sequences
• Frequencies of Restriction Sites
• Isoschizomers
• Time-Saver™ Qualified Enzymes
• Type IIS Restriction Enzymes
• Why Choose Recombinant Enzymes?

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Heat Inactivation
• Megabase Mapping
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases - Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Star Activity
• Traditional Cloning Quick Guide

• Restriction Enzyme Troubleshooting Guide

• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter V2.0
• NEBioCalculator
• REBASE

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0600S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0600S_L_v1_0071301
• R0600S_L_v1_0071306
• R0600S_L_v1_0071312
• R0600S_L_v1_0071405
• R0600S_L_v1_0071410
• R0600S_L_v1_0071509
• R0600S_L_v1_0071605
• R0600S_L_v1_0071702
• R0600S_L_v1_0071708
• R0600S_L_v1_0071802

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• BmrI
• NEBuffer™ 2.1

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.