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Home Restriction Endonucleases Products NaeI

NaeI

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GCC_^GGC

Isoschizomers | Single Letter Code | Pronunciation:

100% activity in rCutSmart^™ Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
Restriction Enzyme Cut Site: GCC/GGC

Catalog #	Concentration	Size	List Price	Your Price
R0190S	10,000 units/ml	500 units	$69.00	Sign In Sign In
R0190L	10,000 units/ml	2,500 units	$281.00	Sign In Sign In

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Product Information

Product Source

An E. coli strain that carries the NaeI gene from Nocardia aerocolonigenes (ATCC 23870).

This product is related to the following categories:: Restriction Endonucleases: N-O

This product can be used in the following applications:: Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0190S -20

NaeI R0190SVIAL -20 1 x 0.05 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

R0190L -20

NaeI R0190LVIAL -20 1 x 0.25 ml 10,000 units/ml

rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 37°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 25%
NEBuffer™ r2.1: 25%
NEBuffer™ r3.1: <10%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation
No
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Isoschizomers

KroNI
MroNI
NgoMIV
PdiI
Related Products
Companion Products
Materials Sold Separately
- rCutSmart™ Buffer
Product Notes
1. Demonstrates marked site preferences and cuts pBR322 DNA very slowly.
2. Inhibited by salt concentrations > 50mM.
3. NgoMIV, an isoschizomer of NaeI, exhibits less site preference.
4. For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.
5. Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.
6. Blocked by CpG methylation.