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Home Restriction Endonucleases Products HgaI

HgaI

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

NEB restriction endonuclease that recognizes the sequence GACGCNNNNN^NNNNN_

Isoschizomers | Single Letter Code | Pronunciation:

Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Restriction Enzyme Cut Site: GACGC(5/10)

Catalog #	Concentration	Size	List Price	Your Price
R0154S	2,000 units/ml	100 units	$75.00	Sign In Sign In

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Product Information

Product Source

A E. coli strain that carries the HgaI gene from Haemophilus gallinarum (ATCC 14385)

This product is related to the following categories:: Restriction Endonucleases H M

This product can be used in the following applications:: Restriction Enzyme Digestion

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

R0154S -20

HgaI R0154SVIAL -20 1 x 0.05 ml 2,000 units/ml

NEBuffer™ r1.1 B6001SVIAL -20 1 x 1.25 ml 10 X

Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of ΦX174 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Reaction Conditions

1X NEBuffer™ r1.1
Incubate at 37°C

1X NEBuffer™ r1.1
10 mM Bis-Tris-Propane-HCl
10 mM MgCl₂
100 µg/ml Recombinant Albumin
(pH 7 @ 25°C)

Activity in NEBuffers
NEBuffer™ r1.1: 100%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 25%
rCutSmart™ Buffer: 100%

Diluent Compatibility
- Diluent A
Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
200 µg/ml BSA
pH 7.4 @ 25°C

Heat Inactivation
65°C for 20 minutes
Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Isoschizomers

CseI
Related Products
Companion Products
Product Notes
1. Of the over 3,000 known restriction endonucleases, HgaI is one of the few that produces extensions of more than 4 bases.
2. Overdigestions with > 5 units of HgaI per µg of DNA is not recommended.
3. HgaI loses activity in the absence of substrate and is essentially inactive after one hour at 37°C.
4. Based on the stability of the enzyme in the reaction, incubations longer than 1 hr will not result in improved digestion, unless additional enzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.
5. Blocked by CpG methylation.
6. star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%