SP6 RNA Polymerase
SP6 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the SP6 promoter.
- Radiolabeled RNA probe preparation
- RNA generation for studies of RNA structure, processing and catalysis
- Expression control via anti-sense RNA
Avoiding RNase Contamination
Bacteriophage SP6 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the SP6 phage promoter. The 98.5 KD polymerase catalyzes in vitro RNA synthesis from a cloned DNA template under the SP6 promoter. RNA synthesized using the SP6 RNA Polymerase is suitable for many applications in research and biotechnology.
1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the SP6 RNA Polymerase promoter. Incubate at 37°C.
Product SourceAn E. coli strain that carries the cloned gene for SP6 RNA Polymerase from Salmonella typhimurium LT2Z.
The following reagents are supplied with this product:
Store at (°C) Concentration RNAPol Reaction Buffer -20 10 X
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