T7 RNA Polymerase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0251S     -20       T7 RNA Polymerase M0251SVIAL -20 1 x 0.1 ml 50,000 units/ml   RNAPol Reaction Buffer B9012SVIAL -20 1 x 1 ml 10 X
M0251L     -20       T7 RNA Polymerase M0251LVIAL -20 1 x 0.5 ml 50,000 units/ml   RNAPol Reaction Buffer B9012SVIAL -20 1 x 1 ml 10 X

Application Features

• Radiolabeled RNA probes
• Non-isotopic RNA labeling
• Preparation of RNA vaccines
• Guide RNA for gene targeting
• mRNA for in vitro translation and micro injection 
• RNA structure, processing and catalysis studies
• RNA amplification
• Anti-sense RNA for gene expression experiment 

Companion Products

• HiScribe^® T7 High Yield RNA Synthesis Kit
• ssRNA Ladder
• RNase Inhibitor, Murine
• Pyrophosphatase, Inorganic (E. coli)
• RNA Loading Dye, (2X)
• E. coli Poly(A) Polymerase
• mRNA Cap 2 O Methyltransferase
• Vaccinia Capping System

1. For radio labeled high specific activity RNA probes, the concentration of the radioactive nucleotide should be limited to 6 μM. 
2. To protect RNA against ribonuclease, RNase inhibitor (NEB #M0314 or #M0307) should be added to a final concentration of 1 U/ μl. 
3. T7 RNA Polymerase is extremely sensitive to salt inhibition. The overall salt concentration should not exceed 50 mM. 

1. Schenborn, E.T. and Meirendorf, R.C. (1985). Nucl. Acids Res.. 13, 6223-6236.
2. Davanloo, P., Rosenberg, A.H., Dunn, J.J. and Studier, F.W. (1984). Proc. Natl. Acad. Sci. USA. 81, 2035-2039.
3. Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, (2nd Ed.). 10.27-10.37.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd Ed.). 18.82-18.84.
5. Melton, D.A., Kreig, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. and Green, M.R. (1984). Nucl. Acids Res.. 12, 7035-7056.
6. Milligan, J.F., Groebe, D.R., Witherell, G.W. and Uhlenbeck, O.C. (1987). Nucl. Acids Res.. 15, 8783.
7. Noren, C.J. et al. (1990). Nucl. Acids Res.. 18, 83-88.
8. Kreig, P.A. and Melton, D.A. (1984). Nucl. Acids Res.. 12, 7057-7070.

1. Protocol for Standard RNA Synthesis

• Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production

• DNA Polymerase Selection Chart
• RNA Polymerase Selection Chart

1. What is the promoter sequence of T7 RNA Polymerase?
2. Is it possible to start transcription with an A?
3. Does the transcription reaction with T7 RNA Polymerase require a primer?
4. Does T7 RNA Polymerase leave an extra base at the end of a transcript?
5. Will T7 RNA Polymerase work on single stranded substrate?
6. Will T7 RNA Polymerase work on uncut plasmid DNA?
7. Can aberrant RNA be produced when using T7 RNA Polymerase?
8. Can I use T7 RNA Polymerase to make high specific activity radiolabeled probes?
9. What are the main causes of reaction failure using T7 RNA Polymerase?
10. Why is the specific activity of the probe low?
11. How can the yield of RNA be maximized when using T7 RNA Polymerase?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0251S_L_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0251S_L_v1_0091405
• M0251S_L_v2_0091405
• M0251S_L_v2_0131410
• M0251S_L_v2_0131502
• M0251S_L_v2_0131507
• M0251S_L_v3_0131507
• M0251S_L_v2_0131601
• M0251S_L_v3_0181605
• M0251S_L_v3_0191610
• M0251S_L_v3_0201709
• M0251S_L_v3_0231804
• M0251L_v3_10026489
• M0251S_v3_10026487
• M0251L_v3_10035565
• M0251L_v3_10040638
• M0251S_v3_10043729
• M0251L_v3_10046341
• M0251S_v3_10047697
• M0251L_v3_10054655
• M0251L_v3_10059617
• M0251S_v3_10054654
• M0251L_v3_10066792
• M0251S_v3_10062698
• M0251L_v3_10069544
• M0251L_v3_10072895
• M0251L_v3_10075394
• M0251L_v3_10078295
• M0251S_v3_10062958
• M0251S_v3_10091328
• M0251L_v3_10083364
• M0251L_v3_10091331
• M0251S_v3_10098431
• M0251L_v3_10101789
• M0251L_v3_10104466
• M0251S_v3_10106627
• M0251S_v3_10109377
• M0251L_v3_10109416
• M0251L_v3_10113865
• M0251S_v3_10113867
• M0251L_v3_10122508
• M0251L_v3_10126417
• M0251S_v3_10133353
• M0251L_v3_10136947
• M0251S_v3_10144366
• M0251L_v3_10149083
• M0251S_v3_10149084
• M0251L_v3_10158543
• M0251L_v3_10169533
• M0251S_v3_10174196
• M0251L_v3_10176998
• M0251L_v3_10199351
• M0251S_v3_10207430
• M0251L_v3_10204912
• M0251S_v3_10225486
• M0251L_v3_10215191
• M0251L_v3_10240120
• M0251L_v3_10249278
• M0251S_v3_10254516
• M0251L_v3_10255340
• M0251L_v3_10274795
• M0251S_v3_10275040
• M0251L_v3_10287464
• M0251S_v3_10288444

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T7 RNA Polymerase
• RNAPol Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.