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  • Ribonucleotide Solution Mix

    Description

    Ribonucleotide Solution Mix is a buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP, pH 7.5 as sodium salts. The NTPs are also available as Ribonucleotide Solution Set (NEB #N0450).

    Supplied as: Sodium salts, pH 7.5

    Reagents Supplied (small):

    NTP Mix (25 mM of each)   0.4 ml

    Reagents Supplied (large): 
    NTP Mix (25 mM of each)   5 x 0.4 ml

    Diluent Compatibility:
    Can be diluted using nuclease-free water

    Advantages and Features

    Applications

    • In vitro transcription

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. Store at -20°C. Avoid multiple freeze/thaw cycles. Dilute with nuclease-free water when necessary.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Assurance Statement

    • Ribonucleotides are certified free of detectable ribonuclease activity. Functional purity is determined by preparative in vito transcription reactions.

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 16 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • RNase Activity (16 Hour Digestion):

      The product is tested in a reaction containing a RNA substrate.  After incubation for 16 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.