RNA Synthesis In vitro Transcription (IVT)
= available in GMP-grade
* “GMP Grade” and “GMP-grade” are branding terms NEB uses to describe products manufactured or finished at NEB’s Rowley facility. The Rowley facility was designed to manufacture products under more rigorous infrastructure and process controls to achieve more stringent product specifications and customer requirements. Products manufactured at NEB’s Rowley facility are manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards. However, at this time, NEB does not manufacture or sell products known as Active Pharmaceutical Ingredients (APIs), nor does NEB manufacture its products in compliance with all of the Current Good Manufacturing Practice regulations.
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- A Typical DNase I Reaction Protocol (M0303)
- Capped RNA Synthesis (E2040)
- Cap-0 synthesis using Vaccinia Capping Enzyme (NEB #M2080)
- DNA Template Preparation (E2040)
- Evaluation of Reaction Products (E2040)
- High Specific Activity Radiolabeled RNA Probe Synthesis (E2040)
- A Typical Tailing Reaction (M0337)
- Purification of Synthesized RNA (E2040)
- SP6 <em>In Vitro</em> Transcription Reaction Protocol (M0207)
- Standard RNA Synthesis (E2040)
- 2´-O-Methylation of Capped RNA (M0366)
- One-Step Cap-1 mRNA synthesis with Vaccinia Capping Enzyme (VCE) and mRNA Cap 2´-O-methyltransferase (2´-O-me) (NEB #M0366, #M2080)
- Labeling Protocol (M2080)
- Capped RNA Synthesis (E2050)
- Purification of Synthesized RNA (E2050)
- Standard RNA Synthesis (E2050)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- Poly(A) Tailing of RNA using E. coli Poly(A) Polymerase (NEB# M0276)
- Protocol for Standard RNA Synthesis (NEB #M0378)
- Evaluation of Reaction Products (E2060)
- mRNA Purification (E2060)
- mRNA Purification (E2065)
- mRNA Synthesis with Modified Nucleotides (E2060)
- mRNA Synthesis with Modified Nucleotides (E2065)
- Protocol for Standard RNA Synthesis
- Standard mRNA Synthesis (E2065)
- Evaluation of Reaction Products (E2065)
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- Transfection of EnGen® Spy Cas9 HF1 (NEB #M0667) into adherent cells using the Lipofectamine® RNAiMAX System
- Purification of Synthesized RNA (E2070)
- Protocol for Standard RNA Synthesis
- RNA Cap Analog Selection Chart
- RNA Ligase Selection Chart
- RNA Polymerase Selection Chart
- Recommended HiScribe® RNA Synthesis Kits by Application
- Avoiding Ribonuclease Contamination
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Usage Guidelines
- Wulf, Madalee; Buswell, John; Chan, Siuhong; Dai, Nan; Marks, Katherine; Tzertzinis, George; Whipple, Joe; Correa, Ivan; Schildkraut, Ira; (2019) The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping Sci Rep; 9 (1), 8594. PubMedID: 31197197, DOI: 10.1038/s41598-019-45083-5
- Wu, M.Z., Asahara, H., Tzertzinis, G., Roy, B. (2020) Synthesis of low immunogenicity RNA with high-temperature in vitro transcription RNA; PubMedID: 31900329
- Potapov, V., Fu, X., Dai, N., Correa, I.R., Jr., Tanner, N.A., Ong, J.L (2018) Base modificatons affecting RNA polymerase and reverse transcriptase fidelity Nucleic Acids Res; 46(11): 5753-5763, PubMedID: 29750267
- Eyler, D.E., Franco, M.K., Batool, Z., Wu, M.Z., Dubuke, M.L., Dobosz-Bartoszek, M., Jones, J.D., Polikanov, Y.S., Roy, B., Koutmou, K.S (2019) Pseudouridinylatio of mRNA coding sequences alters translation Proc Natl Acad Sci U S A; 116(46), 23068-23074.. PubMedID: 31672910, DOI: 10.1073/pnas.1821754116
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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