Home Next Generation Sequencing Library Preparation Products NEBNext^® Ultra^™ II DNA Library Prep with Sample Purification Beads

NEBNext^® Ultra^™ II DNA Library Prep with Sample Purification Beads

Even more from less, for DNA

Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.

Get more of what you need, with the highest library yields
Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
Improve yields and quality for target enrichment applications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats
Enjoy the flexibility and reliability of the gold standard SPRIselect^® size selection and clean-up beads, supplied in just the amounts you need

Note: If your input DNA has not already been sheared, we recommend the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, which fragments, end repairs, and dA-tails DNA, all in a single tube with no cleanups or transfers. This kit is available with and without sample purification beads..

Catalog #	Concentration	Size	List Price	Your Price
E7103S	Not Applicable	24 reactions	$678.00	Sign In Sign In
E7103L	Not Applicable	96 reactions	$2,589.00	Sign In Sign In

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Product Information

The NEBNext Ultra II DNA Library Prep with Sample Purification Beads contains the enzymes, buffers and purification beads required to convert a broad range of DNA input amounts into indexed libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and, for added convenience, this kit contains NEBNext Sample Purification Beads (SPRIselect^® beads from Beckman Coulter) for size selection and enzyme reaction cleanup.

Advantages

Get more of what you need, with the highest library yields
Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
Improve yields and quality for target enrichment applications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats
Enjoy the flexibility and reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need

To learn more about how NEBNext Ultra II addresses lower input amounts and challenging sample types, download our technical note.

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.

This product is related to the following categories:: DNA Library Prep for Illumina,; Automation for NEBNext® NGS Library Prep Products

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

E7103S

Multi-temperature

NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®	E7645S	-20
NEBNext^® Ultra II End Prep Enzyme Mix	E7646AVIAL	-20	1 x 0.072 ml	Not Applicable
NEBNext^® Ultra II End Prep Reaction Buffer	E7647AVIAL	-20	1 x 0.168 ml	Not Applicable
NEBNext^® Ultra^™ II Ligation Master Mix	E7648AVIAL	-20	1 x 0.72 ml	Not Applicable
NEBNext^® Ligation Enhancer	E7374AVIAL	-20	1 x 0.024 ml	Not Applicable
NEBNext^® Ultra II Q5^® Master Mix	E7649AVIAL	-20	1 x 0.6 ml	Not Applicable
NEBNext^® Sample Purification Beads	E7104S	25
NEBNext^® Sample Purification Beads	E7104SVIAL	25	1 x 4 ml	Not Applicable

E7103L

Multi-temperature

NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®	E7645L	-20
NEBNext^® Ultra II End Prep Enzyme Mix	E7646AAVIAL	-20	1 x 0.288 ml	Not Applicable
NEBNext^® Ultra II End Prep Reaction Buffer	E7647AAVIAL	-20	1 x 0.672 ml	Not Applicable
NEBNext^® Ultra^™ II Ligation Master Mix	E7648AAVIAL	-20	3 x 0.96 ml	Not Applicable
NEBNext^® Ligation Enhancer	E7374AAVIAL	-20	1 x 0.096 ml	Not Applicable
NEBNext^® Ultra II Q5^® Master Mix	E7649AAVIAL	-20	2 x 1.2 ml	Not Applicable
NEBNext^® Sample Purification Beads	E7104L	25
NEBNext^® Sample Purification Beads	E7104SVIAL	25	4 x 4 ml	Not Applicable

Properties & Usage
Materials Required but not Supplied
- 80% Ethanol (freshly prepared)
- Nuclease-free Water
- 0.1X TE (1 mM Tris-HCl, pH 8.0, 0.1 mM EDTA)
- DNA LoBind Tubes (Eppendorf #022431021)
- NEBNext Multiplex Oligos for Illumina (for a full list, visit www.neb.com/oligos)
- Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
- PCR Machine
Optional:
- 10 mM Tris-HCl, pH 8.0 or
- Tris/NaCl, pH 8.0 (10 mM Tris, 10 mM NaCl)
  (for adaptor dilution of DNA input ≤ 100 ng)
Storage Temperature:
Package 1: NEBNext Ultra II DNA Library Prep Kit for Illumina - Store at -20°C
Package 2: NEBNext Sample Purification Beads - Store at Room Temperature. Do not freeze.

Protocols, Manuals & Usage
- Protocols
  Where can I find protocols for using NEBNext^® DNA Library Prep reagents?
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
  
  manualE7103-E7645
  
  manualE7103_E7645 w UMI Adaptors DNA
- Application Notes
  Improved Library Preparation with NEBNext^® Ultra™ II DNA Library Prep Kit for Illumina^®
FAQs & Troubleshooting
- FAQs
  For what type of sample prep/library construction can I use the NEBNext Ultra II DNA Library Prep kit for Illumina?
  
  What type and how much starting material do I need to use when preparing libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina?
  
  Is size selection required and what is the recommended method?
  
  Can I use the NEBNext Ultra II DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the Ion Torrent PGM or Proton?
  
  Can NEB provide any of the reagents for the cluster generation step or the actual sequencing run?
  
  Are these reagents available in a customized format, larger format or 96 well formats?
  
  I am currently using Agencourt Ampure XP beads. Will the NEBNext Sample Purification Beads be a suitable replacement?
  
  Which NEBNext Oligos can be used with this library prep kit?
  
  Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?
  
  What do I do if I see a precipitate in the Ultra II End Prep Reaction Buffer?
- Tech Tips
  
  When removing the supernatant from the magnetic beads during cleanup, itis important not to disturb the pellet. Find these and other helpfultips for optimizing the size selection and clean up steps in thisprotocol video.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext^® Ultra^™ II DNA Library Prep with Sample Purification Beads
  
  NEBNext^® Ultra II End Prep Enzyme Mix
  
  NEBNext^® Ultra II End Prep Reaction Buffer
  
  NEBNext^® Ultra^™ II Ligation Master Mix
  
  NEBNext^® Ligation Enhancer
  
  NEBNext^® Ultra II Q5^® Master Mix
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Licenses
  This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
  
  For additional information or to inquire about commercial use, please contact busdev@neb.com.